LOCUS       Exported               10636 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPIPRA561, complete sequence.
ACCESSION   JF811683
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, 
            Zamora P, Alandete-Saez M, Bennett AB.
  TITLE     An intellectual property sharing initiative in agricultural 
            biotechnology: development of broadly accessible technologies for 
            plant transformation
  JOURNAL   Plant Biotechnol. J. 10 (5), 501-510 (2012)
  PUBMED    22221977
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P, 
            Boettiger S, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
            Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 10636)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Binary vector pPIPRA561"
                     /mol_type="other DNA"
                     /db_xref="taxon:1088854"
     misc_feature    7..338
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    245..269
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     gene            350..3380
                     /gene="FMV34S"
                     /label=FMV34S
     regulatory      350..1300
                     /regulatory_class="promoter"
                     /gene="FMV34S"
     CDS             1312..2109
                     /codon_start=1
                     /gene="NPTII"
                     /product="neomycin phosphotransferase II"
                     /label=NPTII
                     /note="confers kanamycin resistance"
                     /protein_id="AEP19806.1"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            1312..2109
                     /gene="NPTII"
                     /label=NPTII
     regulatory      2116..2385
                     /regulatory_class="terminator"
                     /gene="FMV34S"
                     /note="Mas term 3UTR"
     terminator      2127..2379
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      2430..3380
                     /regulatory_class="promoter"
                     /gene="FMV34S"
     misc_feature    3391..3445
                     /label=MCS (polylinker)
                     /note="MCS (polylinker)"
     regulatory      3451..3755
                     /regulatory_class="terminator"
                     /note="pea E9 3 UTR"
     misc_feature    3782..3982
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    3844..3868
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4141..7900
                     /label=Agrobacterium pVS1
                     /note="Agrobacterium pVS1"
     CDS             5320..5949
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6378..7451
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7517..7711
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8038..9175
                     /label=Escherichia coli ColE1
                     /note="Escherichia coli ColE1"
     misc_feature    8185..8325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8511..9099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9564..10358)
                     /codon_start=1
                     /gene="aadA"
                     /product="AadA"
                     /label=aadA
                     /note="confers kanamycin resistance in bacteria"
                     /protein_id="AEP19807.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9564..10358)
                     /gene="aadA"
                     /label=aadA