LOCUS       Exported                3912 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pPICZBBAX, complete sequence.
ACCESSION   LT726836
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Yeast expression vector pPICZBBAX"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 6635."
                     /db_xref="taxon:1945491"
     misc_feature    17..46
                     /label=Myc epitope
                     /note="Myc epitope"
     CDS             17..46
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    62..79
                     /label=His-tag
                     /note="His-tag"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      159..418
                     /regulatory_class="terminator"
                     /note="AOX1 terminator"
     terminator      159..405
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        420..831
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     regulatory      425..830
                     /regulatory_class="promoter"
                     /note="TEF1 promoter"
     regulatory      839..904
                     /regulatory_class="promoter"
                     /note="synthetic EM7 promoter"
     promoter        839..886
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             905..1279
                     /codon_start=1
                     /note="unnamed protein product; zeocin resistance"
                     /protein_id="SJL86637.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1325..1595
                     /regulatory_class="terminator"
                     /note="CYC1 terminator"
     terminator      1345..1592
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1605..1885)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(1667..2255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2337..3275
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     regulatory      2342..3107
                     /regulatory_class="promoter"
                     /note="AOX1 promoter"
     misc_feature    3108..3272
                     /label=5 UTR of AOX1
                     /note="5 UTR of AOX1"
     CDS             3314..3892
                     /codon_start=1
                     /note="unnamed protein product; mBax"
                     /protein_id="SJL86638.1"
                     /translation="MDGSGEQLGSGGPTSSEQIMKTGAFLLQGFIQDRAGRMAGETPEL
                     TLEQPPQDASTKKLSECLRRIGDELDSNMELQRMIADVDTDSPREVFFRVAADMFADGN
                     FNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLVWIQDQGGWEGLLS
                     YFGTPTWQTVTIFVAGVLTASLTIWKKMG"