LOCUS       Exported                4678 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pPICBT3, complete sequence.
ACCESSION   EU196040
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 4678)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Transformation vector pPICBT3"
                     /mol_type="other DNA"
                     /label=used for insertional mutagenesis in Ulva mutabilis
                     /note="used for insertional mutagenesis in Ulva mutabilis"
                     /db_xref="taxon:492501"
     regulatory      1..546
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
     CDS             join(547..554,2051..2417)
                     /codon_start=1
                     /gene="ble"
                     /product="bleomycin resistance protein"
                     /label=ble
                     /note="derived from Streptoalloteichus hindustanus; adapted
                     to C. reinhardtii and Ulva mutabilis codon usage"
                     /protein_id="ABY60798.1"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            547..2417
                     /gene="ble"
                     /label=ble
     intron          555..717
                     /gene="ble"
                     /number=1
                     /note="derived from Ulva RbcS"
     CDS             complement(862..1521)
                     /codon_start=1
                     /gene="Cm"
                     /gene_synonym="CAT"
                     /product="chloramphenicol acetyl transferase"
                     /label=Cm
                     /note="Cm resistance gene from pBR328"
                     /protein_id="ABY60799.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(862..1521)
                     /gene="Cm"
                     /gene_synonym="CAT"
                     /label=Cm
     promoter        complement(1522..1624)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     intron          1718..2050
                     /gene="ble"
                     /number=1
                     /note="derived from Ulva RbcS"
     regulatory      2418..2806
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
     misc_feature    2807..4678
                     /note="derived from cloning vector pUC18 with minor 
                     modifications and deletions"
     promoter        2894..2998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2999..3859
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4030..4618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"