LOCUS       Exported                3860 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPha-NR, complete sequence.
ACCESSION   JN180663
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Stork S, Moog D, Przyborski JM, Wilhelmi I, Zauner S, Maier UG.
  TITLE     Distribution of the SELMA Translocon in Secondary Plastids of Red 
            Algal Origin and Predicted Uncoupling of Ubiquitin-Dependent 
            Translocation from Degradation
  JOURNAL   Eukaryotic Cell 11 (12), 1472-1481 (2012)
  PUBMED    23042132
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Inducible shuttle vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3860)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2011) Cell Biology, Philipps-University Marburg, 
            Karl-von-Frisch Str. 8, Marburg 35032, Germany
REFERENCE   4  (bases 1 to 3860)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Shuttle vector pPha-NR"
                     /mol_type="other DNA"
                     /db_xref="taxon:1114286"
     regulatory      1..422
                     /regulatory_class="promoter"
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
     misc_feature    423..497
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      498..770
                     /regulatory_class="terminator"
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
     regulatory      771..956
                     /regulatory_class="promoter"
                     /note="fcpB; derived from Phaeodactylum tricornutum"
     CDS             957..1331
                     /codon_start=1
                     /product="zeocin resistance protein"
                     /label=zeocin resistance protein
                     /note="ble"
                     /protein_id="AET83762.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1331..1573
                     /regulatory_class="terminator"
                     /note="fcpA; derived from Phaeodactylum tricornutum"
     promoter        complement(1583..1601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1859..2447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2618..3478)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AET83763.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3479..3583)
                     /gene="bla"
                     /label=AmpR promoter