LOCUS       Exported                4635 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPha-DUAL-[2xNR], complete sequence.
ACCESSION   JN180664
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Inducible shuttle vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2011) Cell Biology, Philipps-University Marburg, 
            Karl-von-Frisch Str. 8, Marburg 35032, Germany
REFERENCE   3  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Shuttle vector pPha-DUAL-[2xNR]"
                     /mol_type="other DNA"
                     /db_xref="taxon:1114287"
     regulatory      36..451
                     /regulatory_class="promoter"
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
     misc_feature    452..472
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      473..737
                     /regulatory_class="terminator"
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
     regulatory      782..1197
                     /regulatory_class="promoter"
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
     misc_feature    1198..1272
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      1273..1537
                     /regulatory_class="terminator"
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
     regulatory      1546..1731
                     /regulatory_class="promoter"
                     /note="fcpB; derived from Phaeodactylum tricornutum"
     CDS             1732..2106
                     /codon_start=1
                     /product="zeocin resistance protein"
                     /label=zeocin resistance protein
                     /note="ble"
                     /protein_id="AET83764.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2107..2348
                     /regulatory_class="terminator"
                     /note="fcpA"
     promoter        complement(2358..2376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2634..3222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3393..4253)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AET83765.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4254..4358)
                     /gene="bla"
                     /label=AmpR promoter