LOCUS       Exported                4988 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPERDBY, complete sequence.
ACCESSION   KR072508
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Yagnik B, Padh H, Desai P.
  TITLE     Construction of a new vector for DNA delivery into mammalian cells 
            using non invasive Lactococcus lactis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Yagnik B, Padh H, Desai P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2015) Cell and Molecular Biology, B. V. Patel 
            Pharmaceutical Education Research and Development (PERD) Centre, 
            S.G. Highway, Thaltej, Ahmedabad, Gujarat 380054, India
REFERENCE   3  (bases 1 to 4988)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Mega v. 6
            Sequencing Technology :: Applied Biosystems 3730xl DNA Analyzer 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Expression vector pPERDBY"
                     /mol_type="other DNA"
                     /db_xref="taxon:1717188"
     CDS             complement(201..212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1980..2630
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="ALE71328.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            1980..2630
                     /gene="cat"
                     /label=cat
     enhancer        3311..3614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3615..3818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3926..3935
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3932..4651
                     /codon_start=1
                     /gene="egfp"
                     /product="enhanced green fluorescent protein"
                     /label=egfp
                     /note="EGFP"
                     /protein_id="ALE71327.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            3932..4651
                     /gene="egfp"
                     /label=egfp
     polyA_signal    4774..4896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"