LOCUS       Exported                3098 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPAM, complete sequence.
ACCESSION   AY027531
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Rademacher T, Hausler RE, Hirsch HJ, Zhang L, Lipka V, Weier D, 
            Kreuzaler F, Peterhansel C.
  TITLE     An engineered phosphoenolpyruvate carboxylase redirects carbon and 
            nitrogen flow in transgenic potato plants
  JOURNAL   Plant J. 32 (1), 25-39 (2002)
  PUBMED    12366798
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Rademacher TR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2001) Institut Fuer Biologie I, RWTH Aachen, 
            Worringer Weg 1, Aachen 52074, Germany
REFERENCE   3  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Binary vector pPAM"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:155765"
     repeat_region   89..113
                     /label=right border T-DNA repeat
                     /note="right border T-DNA repeat"
     misc_feature    89..113
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      225..934
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      229..937
                     /label=derived from pRK2
                     /note="derived from pRK2"
     promoter        1007..1098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1099..1959
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAK27302.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1099..1959
                     /gene="bla"
                     /label=bla
     rep_origin      2119..2733
                     /label=derived from pMB1
                     /note="derived from pMB1"
     rep_origin      2130..2718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   3026..3050
                     /label=left border T-DNA repeat
                     /note="left border T-DNA repeat"
     misc_feature    3026..3050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"