LOCUS       Exported                7740 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pP3CG, complete sequence.
ACCESSION   HQ873320
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Blazso P.
  TITLE     pP3CG, 3.6kb mouse cyclin C promoter - hrGFP reporter construction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Blazso P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2011) Institute of Genetics, Biological Research 
            Center, Hungarian Academy of Sciences, Temesvari krt. 62, Szeged 
            H6726, Hungary
REFERENCE   3  (bases 1 to 7740)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Reporter vector pP3CG"
                     /mol_type="other DNA"
                     /db_xref="taxon:1055507"
     promoter        25..129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             130..990
                     /codon_start=1
                     /product="b-lactamase"
                     /label=b-lactamase
                     /note="ampicillin resistance"
                     /protein_id="AEK26870.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1138..1805
                     /label=pUC replication origin
                     /note="pUC replication origin"
     rep_origin      1161..1749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2037..2058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2073..2103
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2111..2127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2135..2151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2172..2190
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2221..5801
                     /regulatory_class="promoter"
                     /note="3.6 kb promoter fragment of mouse cyclin C gene"
     CDS             5810..6529
                     /codon_start=1
                     /product="hrGFP"
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
                     /protein_id="AEK26869.1"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     primer_bind     complement(6553..6569)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6608..6626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6639..7022
                     /label=SV40 polyA signal
                     /note="SV40 polyA signal"
     polyA_signal    6900..7021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7056..7072)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7098..7116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7126..7142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7284..7739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      7301..7607
                     /label=f1 (+) replication origin
                     /note="f1 (+) replication origin"