LOCUS       Exported                6598 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pORI198, complete sequence.
ACCESSION   HG974440
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Papadimitriou K, Anastasiou R, Maistrou E, Plakas T, Papandreou NC, 
            Hamodrakas SJ, Ferreira S, Supply P, Renault P, Pot B, Tsakalidou E.
  TITLE     Analysis of the lactococcal plasmid pSMA198 found in Streptococcus 
            macedonicus ACA-DC 198 supports the dairy origin of the species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Papadimitriou K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2014) Papadimitriou K., Food Science and Human 
            Nutrition, Agricultural University of Athens, 75 Iera Odos str., 
            Athens, 117 55, GREECE
REFERENCE   3  (bases 1 to 6598)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Shuttle vector pORI198"
                     /mol_type="other DNA"
                     /note="Escherichia coli - Streptococcus macedonicus shuttle
                     vector"
                     /db_xref="taxon:1492741"
     misc_feature    1..158
                     /label=lac operon of pUC19
                     /note="lac operon of pUC19"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      559..800
                     /label=predicted ori of plasmid pSMA198
                     /note="predicted ori of plasmid pSMA198"
     CDS             833..2026
                     /codon_start=1
                     /gene="rep"
                     /product="Rep protein"
                     /label=rep
                     /note="replication initiation protein of plasmid pSMA198"
                     /protein_id="CDP22284.1"
                     /translation="MSSIPEKQENQKQVLTLNELSKRKVVEHNSLITSIAKMDKTPLKM
                     FELAVSCINTEEPPKDNIVYLSKRDLFAFFKVSDNDKHSRFKEAIEKMQKQAFFQIKEE
                     AGKGFKFKSIVPIPYVEWTDYHDEVKIEFHREIMPYLINLKKNFTQHALSDLAELNSKY
                     SIILYRWLSMNYNQYEHYSAKGGRRAEQVESYQNPSISIKELRIMTDTVNDYERFQSLE
                     TWILKKPLEEINAHTSFNVTYDKIKKGRSIDSIVFHIEKKRCADDNSYKLNDKMYQAEQ
                     AKKEETEDMLAIEAMKNKYTKLLLDNMLLNSYEMTDTAIMAGLQQHVYPLYDELKDLHG
                     IKAVQDHLSYVASKQEAYSKRNIAKYLKKAIEQYLPTVRGSEIENMADLLKKIEGKA"
     gene            833..2026
                     /gene="rep"
                     /label=rep
     CDS             2023..2607
                     /codon_start=1
                     /product="OrfX"
                     /label=OrfX
                     /note="ORFX; orfX of plasmid pSMA198"
                     /protein_id="CDP22285.1"
                     /translation="MSEDLKTIKELADEFNVSKQAVRKRLTEEFRANHVETVTSNGVKT
                     LVVTYTGYMLLKQHFTTSNATGNDKVTDTSNPTTGDLSVVKILEQQLFVKDKQLENKDS
                     QISQMQNLLDQQQRLALQDKKLLEEYKAENDSLKALKMPSQETEFKHLDNQYKDEVNAL
                     KEKLENLQEQLKEKDQELAQEKSKKWYQFWK"
     CDS             3319..4056
                     /codon_start=1
                     /gene="ermB"
                     /product="ErmB protein"
                     /label=ermB
                     /note="erythromycin resistance gene of plasmid pGh9:ISS1"
                     /protein_id="CDP22286.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            3319..4056
                     /gene="ermB"
                     /label=ermB
     operon          4130..4359
                     /operon="lac operon of pUC19"
                     /label=lac operon of pUC19 operon
     primer_bind     complement(4140..4156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4164..4180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4188..4218)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4233..4254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    complement(4527..5141)
                     /label=rep pMB1 of plasmid pUC19
                     /note="rep pMB1 of plasmid pUC19"
     rep_origin      complement(4542..5130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5301..6161)
                     /codon_start=1
                     /gene="bla"
                     /product="Bla protein"
                     /label=bla
                     /note="ampicillin resistance gene of pUC19"
                     /protein_id="CDP22287.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5301..6161)
                     /gene="bla"
                     /label=bla
     promoter        complement(6162..6266)
                     /gene="bla"
                     /label=AmpR promoter