LOCUS       Exported                3734 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pOPT-GST_tjp1aN, complete sequence.
ACCESSION   KR069098
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Fadeev A, Irion U, Nuesslein-Volhard C, Krauss J, Frohnhoefer HG.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2015) Arbeitsgruppe Nuesslein-Volhard, Max Planck 
            Institute for Developmental Biology, Spemannstrasse 35, Tuebingen, 
            Germany 72076, Germany
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Expression vector pOPT-GST_tjp1aN"
                     /mol_type="other DNA"
                     /db_xref="taxon:1649175"
     CDS             complement(259..1074)
                     /codon_start=1
                     /gene="KanR"
                     /product="aminoglycoside 3-phosphotransferase III"
                     /label=KanR
                     /protein_id="AKF43469.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(259..1074)
                     /gene="KanR"
                     /label=KanR
     rep_origin      1196..1784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1970..2109
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        2270..2288
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2362..2384
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2395..3555
                     /codon_start=1
                     /product="GST_tjp1aN fusion protein"
                     /label=GST_tjp1aN fusion protein
                     /protein_id="AKF43470.1"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDSS
                     GSENLYFQGSHMYKKDIYRPNEPVQISQNPMQPNYYQEDKPYRDYDHQPYRYDGGYMEP
                     KARNFDPHFEDSVPPYNDQWGQYDHTSGYEPQTLYEDGLNRLYGHPQSRNDTGYDTGRP
                     RYGKPTSGPIRHDEPPPSRPQYDTQQTSRSPEPPKQQYYDPSQRPS"
     misc_feature    2395..3066
                     /label=Region: GST
                     /note="Region: GST"
     CDS             2395..3048
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     misc_feature    3067..3088
                     /label=TEV cleavage site
                     /note="TEV cleavage site"
     CDS             3067..3087
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    3097..3552
                     /label=Region: tjp1a
                     /note="Region: tjp1a"
     terminator      3656..3703
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"