LOCUS       Exported                3710 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pOmni, complete sequence.
ACCESSION   KY608793
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma Y.
  TITLE     Development of a dual-expression vector facilitated with 
            selection-free PCR recombination cloning strategy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Ma Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) Rongchang Campus, Southwest University, No. 
            2 Tiansheng Road, Beibei District, Chongqing, Chongqing 400715, 
            China
REFERENCE   3  (bases 1 to 3710)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Cloning vector pOmni"
                     /mol_type="other DNA"
                     /db_xref="taxon:1966387"
     enhancer        239..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      248..824
                     /regulatory_class="promoter"
     promoter        619..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        867..885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      867..883
                     /regulatory_class="promoter"
                     /note="T7"
     protein_bind    905..929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             944..966
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      957..961
                     /regulatory_class="ribosome_binding_site"
     misc_feature    970..976
                     /label=Kozak sequence
                     /note="Kozak sequence"
     misc_feature    985..1002
                     /label=6His-tag
                     /note="6His-tag"
     CDS             985..1002
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_structure  1003..1561
                     /note="expression cassette of lysis gene E of coliphage 
                     Phi-X174"
     CDS             1264..1539
                     /codon_start=1
                     /product="protein E"
                     /label=protein E
                     /note="cell lysis"
                     /protein_id="AQX45535.1"
                     /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNLR
                     KTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVQKE"
     polyA_signal    1569..1793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      1570..1794
                     /regulatory_class="polyA_signal_sequence"
     terminator      1796..1843
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1899..2569)
                     /direction=LEFT
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      complement(1958..2543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2714..3574)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicilliin resistance protein"
                     /protein_id="AQX45536.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3575..3679)
                     /gene="bla"
                     /label=AmpR promoter