LOCUS       Exported                7530 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pOK12-vtp-gentR, complete sequence.
ACCESSION   EF488746
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     A System for Site-Specific Genetic Manipulation of the Relapsing 
            Fever Spirochete Borrelia hermsii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky 
            Mountain Laboratories, National Institute of Allergy and Infectious 
            Diseases, National Institutes of Health, 903 S. 4th Street, 
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Suicide vector pOK12-vtp-gentR"
                     /mol_type="other DNA"
                     /db_xref="taxon:456498"
     rep_origin      20..612
                     /note="P15A origin of replication; derived from Escherichia
                     coli"
     rep_origin      complement(51..595)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             805..1614
                     /codon_start=1
                     /gene="kanR"
                     /product="kanamycin resistance protein"
                     /label=kanR
                     /protein_id="ABS71238.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            805..1614
                     /gene="kanR"
                     /label=kanR
     misc_feature    complement(1727..2512)
                     /gene="thyX"
                     /note="similar to thymidylate synthase; orfZ; 3 end of 
                     gene is not cloned into the vector"
     gene            complement(1727..2512)
                     /gene="thyX"
                     /label=thyX
     CDS             complement(2796..3686)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orfY"
                     /protein_id="ABS71239.1"
                     /translation="MKKSILTVCMFILLCLLSCDIDALNDLLSKAREKFLEENKNIEGS
                     DHKQEGKEEQVDVVKNMEEGVRQVVQGDPVAPVDDAIPAFQYPQQETIEIEEKDLIPST
                     KEEKEAQEEIEKVKSVLEDSKFDQLIENSRKLQSESKQLESDFYRIFSELQTKLQEQRS
                     LPKINSQTDRAKIQELIKLQNRFNEKRTQIDMLMTQVDAGFNERSSAKYFFEEAEKTLK
                     EAITERLKNALRSSFRRVANYLSGQLSREARRYAENSLSLLESSSGKIGEAMGIRKDIE
                     ELIKEAKSYLSSLVR"
     CDS             complement(4061..4690)
                     /codon_start=1
                     /gene="vtp"
                     /product="variable tick protein"
                     /label=vtp
                     /note="derived from Borrelia hermsii"
                     /protein_id="ABS71240.1"
                     /translation="MKKNTLSAILMTLFLFISCNNGGPELKKGEVTKSDGTVLDLSKIS
                     ANIKNAVTFAASVQEVETLVKSIDELAKAIGQKVNADGLTAEADKNDSLVAGVYQLISD
                     VQGKLTKLEIGASKFAGLKEKVVAAKKGSDDFLTKVKAQHNNLGQSAEAPKAIKKGNAD
                     STKGAEELGKLNTAIDELLTAAKDAVEAAIAELTAAPAKPATPVKP"
     gene            complement(4061..4690)
                     /gene="vtp"
                     /label=vtp
     regulatory      4984..5269
                     /regulatory_class="promoter"
                     /note="flaB promoter derived from Borrelia hermsii"
     CDS             5270..5803
                     /codon_start=1
                     /gene="gentR"
                     /product="gentamicin resistance protein"
                     /label=gentR
                     /protein_id="ABS71241.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            5270..5803
                     /gene="gentR"
                     /label=gentR
     CDS             6063..6593
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orfX"
                     /protein_id="ABS71242.1"
                     /translation="MSFSFKNFKLTKMLFIFLLAACSSLQVEHDKINNKVRIYQYLNEN
                     FELKGVVDHQNNTTQIFLYTKIRNHSIVKQTPLILPDGTKIEGKTSYEYDSKASLGKWV
                     NASSFNLNKAILKKILNEDEHVYNKEDIKVQVGLETLKINKAKIIEFLSKLNAIEKQHT
                     QKQHPDKQNTHKQ"