LOCUS       Exported                3469 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pOJ260, complete sequence.
ACCESSION   GU270843
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Liu G, Ou HY, Wang T, Li L, Tan H, Zhou X, Rajakumar K, Deng Z, He 
            X.
  TITLE     Cleavage of phosphorothioated DNA and methylated DNA by the type IV 
            restriction endonuclease ScoMcrA
  JOURNAL   PLoS Genet. 6 (12), E1001253 (2010)
  PUBMED    21203499
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Liu G, He XY, Deng ZX.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2009) School of Life Science & Biotechnology, 
            Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, 
            P.R. China
REFERENCE   3  (bases 1 to 3469)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Cloning vector pOJ260"
                     /mol_type="other DNA"
                     /label=suicide vector
                     /note="suicide vector"
                     /db_xref="taxon:706444"
     CDS             complement(163..507)
                     /codon_start=1
                     /gene="lacZa"
                     /product="lacZ alpha peptide"
                     /label=lacZa
                     /protein_id="ADB65775.1"
                     /translation="MITNSISRAAADPLESTCSPSLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSPMSSTSGIG
                     SAADAKCRST"
     gene            complement(163..507)
                     /gene="lacZa"
                     /label=lacZa
     primer_bind     423..439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(508..524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    532..548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(556..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    601..622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(910..1498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1702..2505
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="apramycin resistance protein"
                     /label=aac(3)IV
                     /protein_id="ADB65776.1"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     gene            1702..2505
                     /gene="aac(3)IV"
                     /label=aac(3)IV
     CDS             1702..2502
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2945..3056
                     /label=RP4 origin of single-stranded DNA transfer
                     /note="RP4 origin of single-stranded DNA transfer"
     oriT            2947..3056
                     /note="incP origin of transfer"