LOCUS       Exported               10257 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pOEHyg, complete sequence.
ACCESSION   KU743913
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Chen L.
  TITLE     Expression system for Pestalotiopsis microspora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Chen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2016) State Key Program of Microbiology and 
            Department of Microbiology, College of Life Sciences, Nankai 
            University, No. 94 Weijin Road, Tianjin 300071, China
REFERENCE   3  (bases 1 to 10257)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Expression vector pOEHyg"
                     /lab_host="Pestalotiopsis microspora"
                     /mol_type="other DNA"
                     /country="China"
                     /db_xref="taxon:1895022"
     misc_feature    1730..1797
                     /label=right border
                     /note="right border"
     misc_feature    1742..1766
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1829..1845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1850..1868
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1904..2436
                     /label=5 homologous flanking region
                     /note="5 homologous flanking region"
     regulatory      2437..3058
                     /regulatory_class="promoter"
                     /note="Pestalotiopsis microspora actin 1 promoter"
     regulatory      3061..3391
                     /regulatory_class="terminator"
                     /note="Nos terminater"
     regulatory      3392..3744
                     /regulatory_class="promoter"
                     /note="Aspergillus nidulans trpC promoter"
     CDS             3755..4780
                     /codon_start=1
                     /gene="hygR"
                     /product="hygromycin B phosphotransferase"
                     /label=hygR
                     /protein_id="AOO87128.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            3755..4780
                     /gene="hygR"
                     /label=hygR
     misc_feature    4806..5390
                     /label=3 homologous flanking region
                     /note="3 homologous flanking region"
     misc_feature    complement(5399..5456)
                     /label=left border
                     /note="left border"
     misc_feature    5464..5488
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     gene            5836..6807
                     /gene="specR"
                     /label=specR
                     /note="spectinomycin resistance protein"
     CDS             6016..6807
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7051..7639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7825..7965
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      8309..8503
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8569..9636)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10070..442)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWASADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRNGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"