LOCUS       Exported                3737 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mutagenesis vector pNZ5319, complete sequence.
ACCESSION   DQ104847
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Lambert JM, Bongers RS, Kleerebezem M.
  TITLE     Cre-lox-based system for multiple gene deletions and 
            selectable-marker removal in Lactobacillus plantarum
  JOURNAL   Appl. Environ. Microbiol. 73 (4), 1126-1135 (2007)
  PUBMED    17142375
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Lambert JM, Bongers RS, Kleerebezem M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2005) Health and Safety Department, NIZO Food 
            Research, Kernhemseweg 2, Ede, Gld 6718ZB, The Netherlands
REFERENCE   3  (bases 1 to 3737)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Mutagenesis vector pNZ5319"
                     /lab_host="DH5alpha"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli
                     /note="derived from Escherichia coli"
                     /db_xref="taxon:341048"
     CDS             1..651
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AAZ79486.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            1..651
                     /gene="cat"
                     /label=cat
     misc_feature    772..805
                     /label=lox71
                     /note="lox71"
     protein_bind    complement(772..805)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      complement(949..1050)
                     /regulatory_class="terminator"
                     /note="Tlas"
     CDS             complement(1162..1899)
                     /codon_start=1
                     /gene="ery"
                     /product="erythromycin resistance protein"
                     /label=ery
                     /protein_id="AAZ79487.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1162..1899)
                     /gene="ery"
                     /label=ery
     rep_origin      2228..3140
                     /label=ori
                     /note="ori"
     rep_origin      2328..2873
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3285..3311
                     /regulatory_class="terminator"
                     /note="TpepN"
     misc_feature    3505..3538
                     /label=lox66
                     /note="lox66"
     protein_bind    complement(3505..3538)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      3609..3721
                     /regulatory_class="promoter"
                     /note="P32"