LOCUS       Exported                4925 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNZ11, complete sequence.
ACCESSION   FJ804173
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
  TITLE     A method for mating clinical Candida albicans isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2009) IMBS, Massey University, Riddet Road, 
            Palmerston North 4410, New Zealand
REFERENCE   3  (bases 1 to 4925)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Cloning vector pNZ11"
                     /mol_type="other DNA"
                     /db_xref="taxon:691220"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(657..1046)
                     /regulatory_class="terminator"
                     /note="Candida albicans tACT1"
     CDS             complement(1063..1635)
                     /codon_start=1
                     /product="NAT"
                     /label=NAT
                     /note="CaNAT; from plasmid pJK850"
                     /protein_id="ACY78685.1"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     primer_bind     complement(1685..1701)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(1704..2723)
                     /regulatory_class="promoter"
                     /note="Candida albicans PACT1"
     misc_feature    2724..656
                     /label=plasmid backbone pBSKS(+)
                     /note="plasmid backbone pBSKS(+)"
     promoter        complement(2737..2755)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2776..2792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2800..2816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2824..2854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2869..2890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3178..3766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3937..4797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4798..4902)
                     /gene="bla"
                     /label=AmpR promoter