LOCUS       Exported                5567 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Dietzia-Escherichia shuttle vector pNV18Sm, complete sequence.
ACCESSION   GQ495223
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Szvetnik A, Bihari Z, Szabo Z, Kelemen O, Kiss I.
  TITLE     Genetic manipulation tools for Dietzia spp
  JOURNAL   J. Appl. Microbiol. 109 (5), 1845-1852 (2010)
  PUBMED    20666867
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Bihari Z, Szvetnik A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2009) Dep. of Appl. Microbiol., BAYBIO, Derkovits 
            fasor 2., Szeged, Csongrad H-6726, Hungary
REFERENCE   3  (bases 1 to 5567)
  AUTHORS   Bihari Z, Szvetnik A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) Dep. of Appl. Microbiol., BAYBIO, Derkovits 
            fasor 2., Szeged, Csongrad H-6726, Hungary
REFERENCE   4  (bases 1 to 5567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Feb 23, 2011 this sequence version replaced GQ495223.1.
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Dietzia-Escherichia shuttle vector pNV18Sm"
                     /mol_type="other DNA"
                     /note="derived from Nocardia-Escherichia coli shuttle 
                     vector pNV18"
                     /db_xref="taxon:672192"
     CDS             859..1650
                     /codon_start=1
                     /gene="AAD"
                     /product="streptomycin/spectinomycin adenyltransferase"
                     /label=AAD
                     /protein_id="ACV66987.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            859..1650
                     /gene="AAD"
                     /label=AAD
     rep_origin      2273..2861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3149..3170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3185..3215
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3223..3239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3247..3263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3259..3507
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase alpha fragment"
                     /label=lacZ
                     /protein_id="ACV66988.1"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDKLA"
     gene            3259..3507
                     /gene="lacZ"
                     /label=lacZ
     misc_feature    3273..3329
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3333..3349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3896..4822
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="ACV66989.1"
                     /translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEANP
                     TALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEYAR
                     RKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAELGAN
                     MPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAEC
                     HARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAIS
                     RKGAAARTAASTVARRAKSASAMEALL"
     gene            3896..4822
                     /gene="repA"
                     /label=repA
     CDS             4819..5154
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /note="replication protein"
                     /protein_id="ACV66990.2"
                     /translation="MSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQERSEWL
                     AEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAAQKAHNE
                     ADNPPLF"
     gene            4819..5154
                     /gene="repB"
                     /label=repB