LOCUS       Exported                4411 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pNV18 DNA, complete sequence.
ACCESSION   AB267085
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Chiba K, Hoshino Y, Ishino K, Kogure T, Mikami Y, Uehara Y, Ishikawa
            J.
  TITLE     Construction of a pair of practical Nocardia-Escherichia coli 
            shuttle vectors
  JOURNAL   Jpn. J. Infect. Dis. 60 (1), 45-47 (2007)
  PUBMED    17314425
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Ishikawa J, Chiba K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2006) Contact:Jun Ishikawa National Institute of 
            Infectious Diseases, Department of Bioactive Molecules; 1-23-1 
            Toyama, Shinjuku, Tokyo 162-8640, Japan URL 
            :http://nocardia.nih.go.jp/
REFERENCE   3  (bases 1 to 4411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Dec 18, 2007 this sequence version replaced AB267085.1.
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shuttle vector pNV18"
                     /mol_type="other DNA"
                     /db_xref="taxon:396412"
     misc_feature    1..2661
                     /label=derived from pK18
                     /note="derived from pK18"
     primer_bind     152..168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    172..228
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(238..254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    262..278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(286..316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    331..352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(640..1228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1512..2306)
                     /codon_start=1
                     /gene="neo"
                     /product="aminoglycoside 3-O-phosphotransferase"
                     /label=neo
                     /protein_id="BAF02355.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(1512..2306)
                     /gene="neo"
                     /label=neo
     misc_feature    2662..4411
                     /label=derived from pAL5000
                     /note="derived from pAL5000"
     CDS             complement(2746..3093)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein RepB"
                     /label=repB
                     /protein_id="BAF02356.2"
                     /translation="MSDGYSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQER
                     SEWLAEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAAQK
                     AHNEADNPPLF"
     gene            complement(2746..3093)
                     /gene="repB"
                     /label=repB
     CDS             complement(3090..4016)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein RepA"
                     /label=repA
                     /protein_id="BAF02357.1"
                     /translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEANP
                     TALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEYAR
                     RKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAELGAN
                     MPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAEC
                     HARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAIS
                     RKGAAARTAASTVARRAKSASAMEALL"
     gene            complement(3090..4016)
                     /gene="repA"
                     /label=repA
     CDS             complement(4405..242)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase alpha fragment"
                     /label=lacZ
                     /protein_id="BAF02358.1"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDKLA"
     gene            complement(4405..242)
                     /gene="lacZ"
                     /label=lacZ