LOCUS       Exported                3297 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTT104 DNA, complete sequence.
ACCESSION   AB032066
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Nomura N, Nagase T.
  TITLE     The nucleotide sequence of the pNTT104 vector
  JOURNAL   Published Only in Database (1999)
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Nomura N, Nagase T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-1999) Nobuo Nomura, Kazusa DNA Research Institute,
            Laboratory of Gene Structure 1; 1532-3 Yana, Kisarazu, Chiba 
            292-0812, Japan (E-mail:nomura@kazusa.or.jp, 
            URL:http://www.kazusa.or.jp, Tel:81-438-52-3932, Fax:81-438-52-3931)
REFERENCE   3  (bases 1 to 3297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     pNTT104
            This sequence is derived from the pUC118 sequence mainly compiled by
            changing the polylinker sequence (sequence at the position between 
            231 and 291 of the pUC118) to the 194-bp sequence (sequence at the 
            position between 231 and 426 of the pNTT104) containing multiple 
            restriction enzyme recognition sites. In addition to that, both the 
            ScaI and NarI sites of the pUC118 were eliminated by nucleotide 
            exchanges at the positions 586 (C to T) and 1802 (G to A) of the 
            pNTT104 sequence, respectively.
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Cloning vector pNTT104"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:103691"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    205..462
                     /note="complementary base sequence was reported as 
                     Acc#:D16587; polylinker"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             217..678
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITACLQAPTSCMGPLDLVLVISIGPYVFQHALKKPSPWKLKA
                     SSSNSRPHVSMDPGSTAAVAGTELILAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSE
                     EARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIRQSNHSTRPVAAH"
     primer_bind     complement(426..442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      655..1110
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1392..1496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1497..2357
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"