LOCUS       Exported                3968 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTAP-TSR, complete sequence.
ACCESSION   EU040032
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Human cytomegalovirus protein kinase UL97 forms a complex with the 
            tegument phosphoprotein pp65
  JOURNAL   J. Virol. 81 (19), 10659-10668 (2007)
  PUBMED    17634236
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2007) Biological Chemistry & Molecular 
            Pharmacology, Harvard Medical School, 250 Longwood Ave., Boston, MA 
            02115, USA
REFERENCE   3  (bases 1 to 3968)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Cloning vector pNTAP-TSR"
                     /mol_type="other DNA"
                     /db_xref="taxon:459304"
     misc_feature    61..234
                     /note="IgG binding domain 1; Staphylococcus aureus protein 
                     A gammaglobulin binding domain"
     misc_recomb     213..262
                     /note="50-bp repeat, substrate for Red-recombination (Rec 
                     E/T) to remove AphAI (kanamycin resistance) marker"
     misc_feature    235..262
                     /note="partial IgG binding domain 2; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     misc_feature    263..280
                     /label=I-SceI homing endonuclease recognition site
                     /note="I-SceI homing endonuclease recognition site"
     CDS             404..1219
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_recomb     1251..1300
                     /note="50-bp repeat, substrate for Red-recombination (Rec 
                     E/T) to remove AphAI (kanamycin resistance) marker"
     misc_feature    1251..1272
                     /note="partial IgG binding domain 1; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     misc_feature    1273..1458
                     /note="IgG binding domain 2; Staphylococcus aureus protein 
                     A gammaglobulin binding domain"
     misc_feature    1462..1482
                     /label=spacer region
                     /note="spacer region"
     misc_feature    1483..1503
                     /note="TEV site; Tobacco Etch Virus Protease recognition 
                     site"
     CDS             1483..1503
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    1504..1509
                     /label=spacer region
                     /note="spacer region"
     misc_feature    1510..1545
                     /note="protein C epitope; binds anti-protein C monoclonal 
                     antibody (Roche)"
     promoter        complement(1606..1624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1882..2470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2641..3501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3502..3606)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3952..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"