LOCUS       Exported                3697 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNT1, complete sequence.
ACCESSION   HQ010044
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Vaillancourt K, Bedard N, Bart C, Tessier M, Robitaille G, Turgeon 
            N, Frenette M, Moineau S, Vadeboncoeur C.
  TITLE     Role of galK and galM in galactose metabolism by Streptococcus 
            thermophilus
  JOURNAL   Appl. Environ. Microbiol. 74 (4), 1264-1267 (2008)
  PUBMED    18065633
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Garneau JE, Dupuis ME, Villion M, Romero DA, Barrangou R, Boyaval P,
            Fremaux C, Horvath P, Magadan AH, Moineau S.
  TITLE     The CRISPR/Cas bacterial immune system cleaves bacteriophage and 
            plasmid DNA
  JOURNAL   Nature 468 (7320), 67-71 (2010)
  PUBMED    21048762
REFERENCE   3  (bases 1 to 3697)
  AUTHORS   Garneau JE, Dupuis M-E., Villion M, Romero DA, Barrangou R, Boyaval 
            P, Fremaux C, Horvath P, Magadan AH, Moineau S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2010) Departement de Biochimie, de Microbiologie 
            et de Bioinformatique, Fac Sciences et Genie, Universite Laval, 
            2420, rue de la Terrasse, Quebec, Quebec G1V 0A6, Canada
REFERENCE   4  (bases 1 to 3697)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Cloning vector pNT1"
                     /host="Streptococcus thermophilus"
                     /mol_type="other DNA"
                     /label=derived from pSMQ172
                     /note="derived from pSMQ172"
                     /db_xref="taxon:927196"
     rep_origin      44..144
                     /note="putative double-stranded origin; dso"
     CDS             261..398
                     /codon_start=1
                     /gene="copG"
                     /product="putative CopG protein"
                     /label=copG
                     /protein_id="ADQ42413.1"
                     /translation="MKKRLTITLSDSVLENFEKMAKEMGLSKSAMISVALENYKKGQVK
                     "
     gene            261..398
                     /gene="copG"
                     /label=copG
     CDS             459..1130
                     /codon_start=1
                     /gene="rep"
                     /product="putative REP protein"
                     /label=rep
                     /protein_id="ADQ42411.1"
                     /translation="MAKEKARYFTFLLYPDSIPEDWEMRLELIGVPIAVSPLHDKDLSD
                     IEGQLYKKAHYHVIYVAKNPVTADSVRVRIKRALGEKSVAVVQIVLNIENTYLYLTHES
                     KDAIKKNKYKYDKADIKHINNFDIDRYLTLDAEQKDDILDEVCNIIADHRLANILELKN
                     FVMAKGSEYGLPNMKVINSVLRSHTGLIRLYFDGVYQERKYGNLVPSVDYETGEVLSEE
                     E"
     gene            459..1130
                     /gene="rep"
                     /label=rep
     oriT            1331..1372
                     /label=putative transfer origin
                     /note="putative transfer origin"
     gene            1495..1958
                     /gene="mob"
                     /label=mob
                     /note="nonfunctional"
     misc_feature    1610..1787
                     /gene="mob"
                     /note="pBlueScript KS multiple cloning site; MCS"
     promoter        1619..1637
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1649..1756
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1666..1682
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1716..1732)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1765..1783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      1960..2106
                     /note="putative single-stranded origin; sso"
     CDS             2391..3041
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /note="CAT"
                     /protein_id="ADQ42412.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            2391..3041
                     /gene="cat"
                     /label=cat
     misc_feature    complement(3324..3695)
                     /label=truncated orf4 from pSMQ172
                     /note="truncated orf4 from pSMQ172"