LOCUS       Exported                3148 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Brucella expression vector pNSClpB, complete sequence.
ACCESSION   EU703958
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Seleem MN, Jain N, Alqublan H, Vemulapalli R, Boyle SM, 
            Sriranganathan N.
  TITLE     Activity of native vs. synthetic promoters in Brucella
  JOURNAL   FEMS Microbiol. Lett. 288 (2), 211-215 (2008)
  PUBMED    18811654
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Seleem MN, Sriranganathan N.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2008) ICTAS, Virginia Tech, 1410 Prices Fork Rd., 
            Blacksburg, VA 24061, USA
REFERENCE   3  (bases 1 to 3148)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Brucella expression vector pNSClpB"
                     /mol_type="other DNA"
                     /db_xref="taxon:535312"
     CDS             462..479
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        643..745
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(1493..2155)
                     /codon_start=1
                     /product="Rep"
                     /label=Rep
                     /note="required for plasmid replication"
                     /protein_id="ACD92291.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2156..2925
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"