LOCUS       Exported                7830 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNS-2xMyc, complete sequence.
ACCESSION   KU725771
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Ha H-J.
  TITLE     Construction of a thiamine-repessible vector for yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Ha H-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2016) Department of Horticulture, Chungnam 
            National University, 99 Daehak-ro, Daejeon, Yuseong 305-761, 
            Republic of Korea
REFERENCE   3  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Cloning vector pNS-2xMyc"
                     /mol_type="other DNA"
                     /db_xref="taxon:1891251"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            280..1399
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        280..623
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     misc_feature    624..1196
                     /label=Nac
                     /note="Nac"
     CDS             624..1196
                     /codon_start=1
                     /product="nourseothricin acetyltransferase"
                     /label=nourseothricin acetyltransferase
                     /protein_id="AOE23681.1"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      1202..1399
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     5UTR           2543..2613
     regulatory      2552..2618
                     /regulatory_class="promoter"
                     /note="nmt1; transcriptional starting site (tss)"
     misc_feature    2618..2753
                     /label=multiple cloning site (MCS)
                     /note="multiple cloning site (MCS)"
     CDS             2624..2689
                     /codon_start=1
                     /product="2x Myc epitope"
                     /label=2x Myc epitope
                     /protein_id="AOE23682.1"
                     /translation="MEQKLISEEDLGEQKLISEEDL"
     CDS             2627..2656
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2660..2689
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2757..2898
                     /regulatory_class="terminator"
                     /note="Tnmt1"
     misc_feature    3752..4958
                     /note="autonomously replicating sequence 1; ars1"
     rep_origin      3825..4609
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(4959..4975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5188..5643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5925..6029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6030..6890
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AOE23683.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            6030..6890
                     /gene="bla"
                     /label=bla
     rep_origin      7061..7649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"