LOCUS       Exported                6069 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNRSAL, complete sequence.
ACCESSION   AF515711
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Park HH, Lim WK, Shin HJ.
  TITLE     In vitro binding of purified NahR regulatory protein with promoter 
            Psal
  JOURNAL   Biochim. Biophys. Acta 1725 (2), 247-255 (2005)
  PUBMED    15978733
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Park HH, Lim WK, Shin HJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2002) Dongseo University, San 69-1, Jurye -2-dong,
            Sasang-gu, Busan 617-716, Korea
REFERENCE   3  (bases 1 to 6069)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Cloning vector pNRSAL"
                     /mol_type="other DNA"
                     /db_xref="taxon:348104"
     CDS             complement(233..1135)
                     /codon_start=1
                     /product="NahR"
                     /label=NahR
                     /note="transcriptional activator"
                     /protein_id="AAP46188.1"
                     /translation="MELRDLDLNLLVVFNQLLVDRRVSITAENLGLTQPAVSNALKRLR
                     TSLQDPLFVRTHQGMEPTPYAAHLAEPVTSAMHALRNALQHHESFDPLTSERTFTLAMT
                     DIGEIYFMPRLMDVLAHQAPNCVISTVRDSSMSLMQALQNGTVDLAVGLLPNLQTGFFQ
                     RRLLQNHYVCLCRKDHPVTREPLTLERFCSYGHVRVIAAGTGHGEVDTYMTRVGIRRDI
                     RLEVPHFAAVGHILQRTDLLATVPIRLADCCVEPFGLSALPHPVVLPEIAINMFWHAKY
                     HKDLANIWLRQLMFDLFTD"
     regulatory      complement(1136..1225)
                     /regulatory_class="promoter"
                     /note="Pr promoter"
     regulatory      1214..1282
                     /regulatory_class="promoter"
                     /note="Psal promoter"
     regulatory      1333..1342
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1339..2991
                     /codon_start=1
                     /product="firefly luciferase protein"
                     /label=firefly luciferase protein
                     /note="reporter gene; derived from cloning vector 
                     pGL3-basic from GenBank Accession Number U47295"
                     /protein_id="AAP46189.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3032..3153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3572..4160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4331..5191)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance"
                     /protein_id="AAP46190.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5192..5296)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5323..5778
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    5909..5957
                     /note="synthetic polyadenylation signal"
     misc_feature    5971..6062
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"