LOCUS       Exported                8522 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expresssion vector pNOV108, complete sequence.
ACCESSION   KX580601
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Richie DL, Takeoka KT, Bojkovic J, Metzger LE IV, Rath CM, Sawyer 
            WS, Wei JR, Dean CR.
  TITLE     Toxic Accumulation of LPS Pathway Intermediates Underlies the 
            Requirement of LpxH for Growth of Acinetobacter baumannii ATCC 19606
  JOURNAL   PLoS ONE 11 (8), E0160918 (2016)
  PUBMED    27526195
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2016) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Expresssion vector pNOV108"
                     /mol_type="other DNA"
                     /db_xref="taxon:1892595"
     rep_origin      7..1337
                     /label=derived from Acinetobacter ATCC23237
                     /note="derived from Acinetobacter ATCC23237"
     CDS             1581..2672
                     /codon_start=1
                     /gene="lacI"
                     /product="LacI"
                     /label=lacI
                     /note="lactose operon repressor; derived from Acinetobacter
                     baumannii ATCC19606"
                     /protein_id="AOF80292.1"
                     /translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAM
                     AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER
                     SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGD
                     WSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTED
                     SSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTA
                     SPRALADSLMQLARQVSRLESGQ"
     gene            1581..2672
                     /gene="lacI"
                     /label=lacI
     CDS             1590..2672
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             complement(2781..5432)
                     /codon_start=1
                     /gene="alaS"
                     /product="AlaS"
                     /EC_number="6.1.1.7"
                     /label=alaS
                     /note="alanyl-tRNA synthetase; derived from Acinetobacter 
                     baumannii"
                     /protein_id="AOF80293.1"
                     /translation="MSTRLMTSAEIREAFLRYFETQGHTRVASSSLVPANDPTLLFTNA
                     GMNQFKDCFLGLEKRDYVRATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGD
                     YFKRDALKFAWEFLTSEQWLALPKDKLYVTVYHTDDEAYDIWNKEIGLAPERIIRIGDN
                     KGEKYASDNFWAMGDTGPCGPCSEIFFDHGEHIWGGLPGSPEEDGDRFIEIWNNVFMQF
                     NRTADGVLHPLPAPSVDTGMGLERISAVLQHVNSNYDIDLFQHLLKAAANIIGIEDEGQ
                     PSLRVVADHARSCCFLIADGVNPSNEGRGYVLRRIIRRAVRHGNKLGATGTFFYKMLQP
                     LIEVMGQAYPELEARREVIEATLIREEEQFAKTLEQGLKLLEGELAQLKDKTIPGATVF
                     KLYDTYGFPTDLTADIARERGFIIDEAGFEVEMAAQRQRARDAGKFAVDYNNIVKVEGE
                     TQFDGYTNTTGQGQIVAIYKDGVQVDEVSEGDEALIVLNQTPFYAESGGQIGDTGIFKN
                     ETGIFEVQDTKKSGGAFVHQGIVTVGNLKTSQNVEAIVKADIREATARNHSATHLLHAA
                     LRQILGSHVQQKGSLVASDILRFDFANDQPVSFEQLQQVERLVNAEIIANTAVTTELLD
                     IETAKAKGAMMLFGEKYGDEVRVLSMGSVIDEKNFSIELCGGIHVKRTGDIGLFKITSE
                     GGVAAGVRRIEAVTGTKALEVVQKADHDIQHINSLLKAQKDQTVERVQANVELVSALQK
                     QIEQLNQKLANFQAADLIDQVQTIAGRQTLITTVQGVDAKALRNLHDSVKSKLENAVIV
                     LAGVEGDKVSLIASVASQYTANLKAGDIIKHLATELGGKGGGKPDLAQGGAPLNEKFGQ
                     VMAALPAWLEQK"
     gene            complement(2781..5432)
                     /gene="alaS"
                     /label=alaS
     promoter        5687..5778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5779..6639
                     /codon_start=1
                     /product="beta-lactamases"
                     /EC_number="3.5.2.6"
                     /label=beta-lactamases
                     /protein_id="AOF80294.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6689..7850
                     /label=derived from Escherichia coli pBR322
                     /note="derived from Escherichia coli pBR322"
     rep_origin      6692..7147
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      7258..7846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8032..8172
                     /label=bom
                     /note="basis of mobility region from pBR322"