LOCUS       Exported               10383 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNOV044, complete sequence.
ACCESSION   KY933088
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Wei J-R.
  TITLE     LpxK is essential for growth of Acinetobacter baumannii ATCC 19606: 
            relationship to toxic accumulation of lipid A pathway intermediates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron Way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Cloning vector pNOV044"
                     /mol_type="other DNA"
                     /db_xref="taxon:2020289"
     regulatory      7..1337
                     /regulatory_class="replication_regulatory_region"
     regulatory      1430..1629
                     /regulatory_class="promoter"
     promoter        7781..7809
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             7998..8531
                     /codon_start=1
                     /gene="accC1"
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /label=accC1
                     /note="confers gentamicin resistance"
                     /protein_id="ASL05636.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            7998..8531
                     /gene="accC1"
                     /label=accC1
     regulatory      8550..9711
                     /regulatory_class="replication_regulatory_region"
     rep_origin      8553..9008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      9119..9707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9893..10033
                     /label=bom
                     /note="basis of mobility region from pBR322"