LOCUS       Exported               11403 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNOV043, complete sequence.
ACCESSION   KY933087
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Wei J-R.
  TITLE     LpxK is essential for growth of Acinetobacter baumannii ATCC 19606: 
            relationship to toxic accumulation of lipid A pathway intermediates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron Way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 11403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Cloning vector pNOV043"
                     /mol_type="other DNA"
                     /db_xref="taxon:2020288"
     regulatory      7..1337
                     /regulatory_class="replication_regulatory_region"
     regulatory      1430..1629
                     /regulatory_class="promoter"
     CDS             1630..2640
                     /codon_start=1
                     /gene="lpxK"
                     /product="tetraacyldisaccharide 4-kinase"
                     /EC_number="2.7.1.130"
                     /label=lpxK
                     /note="LpxK; involved in lipid A biosynthesis"
                     /protein_id="ASL05635.1"
                     /translation="MSLAQLIQNAWNKQSSWLIVLRPLSCLYRAGFLLNRGFYSSGFKK
                     VYTAPVPVMVIGNITVGGSGKTPLLIELVNYLKQHNVKVGVISRGYGGSGPFPMLVTSA
                     SQAAEAGDEPALIVQSTGVPMAVGPNRQAAIELLLASTKLDLIISDDGLQHWALGRQIE
                     WIVLDQNRGLGNRKLLPEGYLREPVERLKTSTVIEHTFTPTTTLHMHLDAGQPYLLNPS
                     SATELSFNIQNNYHAVVGIGFPQRFYQTLKGLGVKQFQEHAFRDHHDYSIDDLLFNDDQ
                     PIITTEKDAVKLLPLLEKHPEFKRPIWVVPVKAVLSTECYQVLNQQLQKLDIQIS"
     gene            1630..2640
                     /gene="lpxK"
                     /label=lpxK
     promoter        8801..8829
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             9018..9551
                     /codon_start=1
                     /gene="accC1"
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /label=accC1
                     /note="confers gentamicin resistance"
                     /protein_id="ASL05634.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            9018..9551
                     /gene="accC1"
                     /label=accC1
     regulatory      9570..10731
                     /regulatory_class="replication_regulatory_region"
     rep_origin      9573..10028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      10139..10727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    10913..11053
                     /label=bom
                     /note="basis of mobility region from pBR322"