LOCUS       Exported               14825 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  HIV-1 vector pNL4-3, complete sequence.
ACCESSION   AF324493
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Adachi A, Gendelman HE, Koenig S, Folks T, Willey R, Rabson A, 
            Martin MA.
  TITLE     Production of acquired immunodeficiency syndrome-associated 
            retrovirus in human and nonhuman cells transfected with an 
            infectious molecular clone
  JOURNAL   J. Virol. 59 (2), 284-291 (1986)
  PUBMED    3016298
REFERENCE   2  (bases 1 to 14825)
  AUTHORS   Bosche WJ, Poon DTK., Ott DE, Hu W-S., Gorelick RJ.
  TITLE     Complete Plasmid Sequence of pNL4-3
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14825)
  AUTHORS   Bosche WJ, Poon DTK., Ott DE, Hu W-S., Gorelick RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2000) AIDS Vaccine Program, SAIC Frederick and 
            Drug Resistance Program, National Cancer Institute at Frederick, PO 
            Box B, Frederick, Maryland 21702-1202, USA
REFERENCE   4  (bases 1 to 14825)
  AUTHORS   Strebel KJ, Martin MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2010) NIAID, Viral Biochemistry, National 
            Institutes of Health, Bethesda, MD, USA
REFERENCE   5  (bases 1 to 14825)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On May 24, 2010 this sequence version replaced AF324493.1.
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="HIV-1 vector pNL4-3"
                     /focus
                     /plasmid="pNL4-3"
                     /mol_type="other DNA"
                     /db_xref="taxon:151458"
     source          1..9709
                     /organism="Human immunodeficiency virus 1"
                     /strain="NY5/BRU (LAV-1)"
                     /mol_type="other DNA"
                     /proviral
                     /note="from sequence deposited in GenBank Accession Number 
                     M19921"
                     /db_xref="taxon:11676"
     repeat_region   1..634
                     /label=5 long terminal repeat
                     /note="5 long terminal repeat"
     LTR             377..634
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     repeat_region   454..550
                     /note="R; 5 copy"
     CDS             790..2292
                     /codon_start=1
                     /product="gag polyprotein"
                     /label=gag polyprotein
                     /protein_id="AAK08483.1"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2085..5096
                     /codon_start=1
                     /product="pol polyprotein"
                     /label=pol polyprotein
                     /protein_id="AAK08484.2"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_difference 2421
                     /replace="g"
                     /note="compared to sequence deposited in GenBank Accession 
                     Number M19921; results in arginine to glycine substitution"
     misc_feature    4781..4898
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             5041..5619
                     /codon_start=1
                     /product="vif protein"
                     /label=vif protein
                     /protein_id="AAK08482.1"
                     /translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHYE
                     STNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDLAD
                     QLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIKPPL
                     PSVRKLTEDRWNKPQKTKGHRGSHTMNGH"
     CDS             5559..5849
                     /codon_start=1
                     /product="vpr protein"
                     /label=vpr protein
                     /protein_id="AAK08485.1"
                     /translation="MEQAPEDQGPQREPYNEWTLELLEELKSEAVRHFPRIWLHNLGQH
                     IYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTRQRRARNGASRS"
     misc_feature    5743..5748
                     /label=EcoRI site of recombination
                     /note="EcoRI site of recombination"
     CDS             join(5830..6044,8369..8414)
                     /codon_start=1
                     /product="tat protein"
                     /label=tat protein
                     /protein_id="AAK08486.1"
                     /translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALGI
                     SYGRKKRRQRRRAHQNSQTHQASLSKQPTSQSRGDPTGPKE"
     CDS             join(5969..6044,8369..8643)
                     /codon_start=1
                     /product="rev protein"
                     /label=rev protein
                     /protein_id="AAK08487.1"
                     /translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTVLESGTKE"
     CDS             6061..6306
                     /codon_start=1
                     /product="vpu protein"
                     /label=vpu protein
                     /protein_id="AAK08488.1"
                     /translation="MQPIIVAIVALVVAIIIAIVVWSIVIIEYRKILRQRKIDRLIDRL
                     IERAEDSGNESEGEVSALVEMGVEMGHHAPWDIDDL"
     CDS             6221..8785
                     /codon_start=1
                     /product="envelope polyprotein"
                     /label=envelope polyprotein
                     /protein_id="AAK08489.2"
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPVW
                     KEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVEQM
                     HEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFNIST
                     SIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFA
                     ILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANFTDNAKT
                     IIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNATL
                     KQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWFNSTWS
                     TEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGN
                     NNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNMTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVNGSLAL
                     IWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVNL
                     LNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGLERILL"
     misc_feature    7759..7992
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8515..8541
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             8787..9407
                     /codon_start=1
                     /product="nef protein"
                     /label=nef protein
                     /protein_id="AAK08490.1"
                     /translation="MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAITS
                     SNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ
                     RRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTS
                     LLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC"
     repeat_region   9076..9709
                     /label=3 long terminal repeat
                     /note="3 long terminal repeat"
     LTR             9076..9709
                     /label=3 LTR
                     /note="3 long terminal repeat (LTR) from HIV-1"
     repeat_region   9529..9626
                     /note="R; 3 copy"
     regulatory      9602..9607
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    9710..11283
                     /label=3 cellular genomic sequence
                     /note="3 cellular genomic sequence"
     rep_origin      complement(11520..12108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12279..13139)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="from pUC vector"
                     /protein_id="AAK08491.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13140..13244)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    13648..14825
                     /label=5 cellular genomic sequence
                     /note="5 cellular genomic sequence"