LOCUS       Exported               12076 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  HIV-1 vector pNL-DT5R DNA, complete sequence.
ACCESSION   AB266485
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Kamada K, Igarashi T, Martin MA, Khamsri B, Hatcho K, Yamashita T, 
            Fujita M, Uchiyama T, Adachi A.
  TITLE     Generation of HIV-1 derivatives that productively infect macaque 
            monkey lymphoid cells
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 103 (45), 16959-16964 (2006)
  PUBMED    17065315
REFERENCE   2  (bases 1 to 12076)
  AUTHORS   Kamada K, Adachi A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2006) Contact:Kazuya Kamada Kobe university school
            of medicine, Department of microbiology; 7-5-1, Kusunokicho, 
            Chuo-ku, Kobe, Hyogo 650-0017, Japan
REFERENCE   3  (bases 1 to 12076)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="HIV-1 vector pNL-DT5R"
                     /mol_type="other DNA"
                     /db_xref="taxon:395074"
     repeat_region   1..636
                     /label=5 long terminal repeat
                     /note="5 long terminal repeat"
     LTR             1..634
                     /label=3 LTR
                     /note="3 long terminal repeat (LTR) from HIV-1"
     repeat_region   454..550
                     /note="R; 5 copy"
     CDS             790..2286
                     /codon_start=1
                     /product="gag polyprotein"
                     /label=gag polyprotein
                     /note="Cyclophilin binding loop (PVHAGPIAP) in capsid 
                     region was replaced to SIVmac analogues (PQPAPQQ)"
                     /protein_id="BAF34641.1"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPQPAPQ
                     QGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSIL
                     DIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPGAT
                     LEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEGHI
                     AKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPE
                     ESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2079..5090
                     /codon_start=1
                     /product="pol polyprotein"
                     /label=pol polyprotein
                     /protein_id="BAF34642.1"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVGQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     VAGDDCVASRQDED"
     misc_difference 2294
                     /replace="a"
                     /compare="AF324493.1"
     misc_feature    4775..4892
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5092..5096
                     /note="artificially inserted sequence for generating Sma I 
                     site"
     CDS             5097..5741
                     /codon_start=1
                     /product="vif protein"
                     /label=vif protein
                     /note="derived from SIVmac239"
                     /protein_id="BAF34643.1"
                     /translation="MEEEKRWIAVPTWRIPERLERWHSLIKYLKYKTKDLQKVCYVPHF
                     KVGWAWWTCSRVIFPLQEGSHLEVQGYWHLTPEKGWLSTYAVRITWYSKNFWTDVTPNY
                     ADILLHSTYFPCFTAGEVRRAIRGEQLLSCCRFPRAHKYQVPSLQYLALKVVSDVRSQG
                     ENPTWKQWRRDNRRGLRMAKQNSRGDKQRGGKPPTKGANFPGLAKVLGILA"
     CDS             5744..6034
                     /codon_start=1
                     /product="vpr protein"
                     /label=vpr protein
                     /protein_id="BAF34644.1"
                     /translation="MEQAPEDQGPQREPYNEWTLELLEELKSEAVRHFPRIWLHNLGQH
                     IYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTRQRRARNGASRS"
     CDS             join(6015..6229,8554..8599)
                     /codon_start=1
                     /product="tat protein"
                     /label=tat protein
                     /protein_id="BAF34645.1"
                     /translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALGI
                     SYGRKKRRQRRRAHQNSQTHQASLSKQPTSQSRGDPTGPKE"
     CDS             join(6154..6229,8554..8828)
                     /codon_start=1
                     /product="rev protein"
                     /label=rev protein
                     /protein_id="BAF34646.1"
                     /translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTVLESGTKE"
     CDS             6246..6491
                     /codon_start=1
                     /product="vpu protein"
                     /label=vpu protein
                     /protein_id="BAF34647.1"
                     /translation="MQPIIVAIVALVVAIIIAIVVWSIVIIEYRKILRQRKIDRLIDRL
                     IERAEDSGNESEGEVSALVEMGVEMGHHAPWDIDDL"
     CDS             6406..8970
                     /codon_start=1
                     /product="envelope polyprotein"
                     /label=envelope polyprotein
                     /protein_id="BAF34648.1"
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPVW
                     KEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVEQM
                     HEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDINTNSSSGRMIMEKGEIKNCSFNIST
                     SIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFA
                     ILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANLTDNAKT
                     IIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNATL
                     KQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWFNSTWS
                     TEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGN
                     NNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGCTSMTLTVQARQLLSDIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNMTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVNGSLAL
                     IWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVNL
                     LNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGLERILL"
     misc_difference 6818
                     /replace="t"
                     /compare="AF324493.1"
     misc_difference 7228
                     /replace="c"
                     /compare="AF324493.1"
     CDS             8700..8726
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             8972..9592
                     /codon_start=1
                     /product="nef protein"
                     /label=nef protein
                     /protein_id="BAF34649.1"
                     /translation="MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAITS
                     SNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ
                     RRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTS
                     LLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC"
     repeat_region   9261..9894
                     /label=3 long terminal repeat
                     /note="3 long terminal repeat"
     LTR             9261..9894
                     /label=3 LTR
                     /note="3 long terminal repeat (LTR) from HIV-1"
     repeat_region   9714..9811
                     /note="R; 3 copy"
     regulatory      9787..9792
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    9895..12076
                     /label=pUC vector
                     /note="pUC vector"
     primer_bind     complement(9919..9935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9943..9959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9967..9997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10012..10033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10321..10909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11080..11940)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="from pUC vector"
                     /protein_id="BAF34650.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11941..12045)
                     /gene="bla"
                     /label=AmpR promoter