LOCUS       Exported                6522 bp ds-DNA     circular SYN 08-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    NGFR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, 
            Pui JC, Koretzky GA, Pear WS
  TITLE     Notch1 regulates maturation of CD4+ and CD8+ thymocytes by 
            modulating TCR signal strength.
  JOURNAL   Immunity. 2001 Mar . 14(3):253-64.
  PUBMED    11290335
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2001 Mar . 14(3):253-64."
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(2..21)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(681..1541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1304..1323
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1542..1646)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1714..1732
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1770..1792)
                     /label=pGEX 3
                     /note="pGEX vectors, reverse primer"
     primer_bind     1892..1911
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2105..2127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2255..2274
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2474..2990
                     /label=5 LTR
                     /note="5 long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3054..3395
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3462..3878
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3808..3830
                     /label=pLXSN 5
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3846..3862
                     /label=pBABE 5
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3924..4476
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4068..4085)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4295..4314
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             5390..5904
                     /label=3 LTR
                     /note="3 long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6066..6082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6090..6120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6135..6156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6273..6290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6444..6522,1..510))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"