LOCUS       Exported                4065 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNG1A, complete sequence.
ACCESSION   JN940926
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E, 
            Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L,
            Munoz-Dorado J.
  TITLE     Comprehensive Set of Integrative Plasmid Vectors for 
            Copper-Inducible Gene Expression in Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 78 (8), 2515-2521 (2012)
  PUBMED    22287008
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Gomez-Santos N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2011) Microbiology, University of Granada (Spain),
            Avda. Fuentenueva s/n, Granada, Granada 18071, Spain
REFERENCE   3  (bases 1 to 4065)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Cloning vector pNG1A"
                     /mol_type="other DNA"
                     /note="optimized for expression in Myxococcus xanthus 
                     DK1622"
                     /db_xref="taxon:1156083"
     regulatory      7..973
                     /regulatory_class="promoter"
                     /note="cuoA promoter"
     misc_feature    974..1008
                     /label=multicloning site
                     /note="multicloning site"
     primer_bind     complement(1021..1037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1045..1061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1069..1099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1114..1135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1423..2011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2492..3286
                     /codon_start=1
                     /gene="kmR"
                     /product="aminoglycoside 3-phosphotransferase"
                     /label=kmR
                     /note="kanamycin resistance protein"
                     /protein_id="AFC76263.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2492..3286
                     /gene="kmR"
                     /label=kmR
     primer_bind     4037..4053
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"