NFkB reporter vector is utilized for detecting NFkB (Nuclear factor of kappa light polypeptide gene) transactivation in a cell-based assay.
| 载体基本信息 | |||
| 载体名称 | pNFkB-Luc | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 5695 bp | 载体类型 | Reporter vector |
| 载体来源 | Zheng C-F. | 筛选标记 | Fluc |
pNFkB-Luc 载体载体图谱
pNFkB-Luc 载体载体序列
LOCUS Exported 5695 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Reporter vector pNFkB-Luc, complete sequence.
ACCESSION AF053315
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5695)
AUTHORS Zheng C-F.
TITLE Direct Submission
JOURNAL Submitted (11-MAR-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 2 (bases 1 to 5695)
AUTHORS Zheng C-F.
TITLE Direct Submission
JOURNAL Submitted (20-JAN-1999) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 3 (bases 1 to 5695)
AUTHORS .
TITLE Direct Submission
COMMENT On Jan 21, 1999 this sequence version replaced gi:2984705.
FEATURES Location/Qualifiers
source 1..5695
/organism="Reporter vector pNFkB-Luc"
/host="Eschericia coli K12"
/mol_type="genomic DNA"
/note="designed for detection of activation of NF-kB signal
transduction pathway"
/db_xref="taxon:74543"
promoter 32..136
/gene="bla"
/label=AmpR promoter
CDS 137..997
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 1934..2074
/label=bom
/note="basis of mobility region from pBR322"
polyA_signal 2313..2445
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 2539..2671
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2757..2788
/label=minP
/note="minimal TATA-box promoter with low basal activity"
CDS 2815..4467
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 4598..4663
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 5233..5367
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
