LOCUS       Exported                9246 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Expression vector pNDC_SUB4, complete sequence.
ACCESSION   LN866853
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Whole gene expression profiles of dermatophytes during infection and
            their virulance traits
  JOURNAL   Thesis (2015) Centre Hospitalier Universitaire Vaudois
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2015) Department of Dermatology, Centre 
            Hospitalier de Lausanne (CHUV), Bugnon 48, 1011 Lausanne, 
            SWITZERLAND
REFERENCE   3  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Expression vector pNDC_SUB4"
                     /lab_host="Arthtoderma benhamiae LAU2354-2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1672048"
     CDS             273..1067
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR; confers resistance to kanamycin"
                     /protein_id="CRN13534.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            273..1067
                     /gene="aphA-3"
                     /label=aphA-3
     CDS             1366..2514
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trans-acting replication protein that binds to
                     /note="trfA"
                     /protein_id="CRN13535.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    2640..2664
                     /note="LB T-DNA repeat; left border repeat from nopaline 
                     C58 T-DNA"
     misc_feature    2698..2722
                     /note="LB T-DNA repeat; left border repeat from nopaline 
                     C58 T-DNA"
     primer_bind     2958..2974
                     /note="M13 fwd; common sequencing primer, one of multiple 
                     similar variants"
     CDS             complement(3843..4868)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=aph(4)-Ia
                     /note="HygR; confers resistance to hygromycin"
                     /protein_id="CRN13536.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            complement(3843..4868)
                     /gene="aph(4)-Ia"
                     /label=aph(4)-Ia
     CDS             complement(6263..7486)
                     /codon_start=1
                     /product="subtilisin-like protease SUB4"
                     /label=subtilisin-like protease SUB4
                     /note="sub4"
                     /protein_id="CRN13537.1"
                     /translation="MVCLKTLSVFLAAFAAADARAVFKTQGHKNSEMIPDNYIVVMKDG
                     VSQDDFKAHISSVASIHSTNKAKRGTNTQGMKREFDIMNWRGYHGHFDRDTLEEILNDS
                     KVDYVEQDQVVRISGLVTQRSAPSWGLGRVSHRQAGSRDYVFDDSAGRGVTIYGVDTGI
                     DINHQDFRGRARWGTNTADRDNADRHGHGTHTASTFAGTAYGIAKNANIVAVKVLGSDG
                     SGSTSGIIAGINYCVQDAQQRGILGKAAMNLSLGGGFSQANNDAVTRAQNAGIFVAVAA
                     GNDNRDARNYSPASAPAVCTVASSTINDSKSSFSNWGPVELKANITSVDIYAPGSDIIA
                     ARPGGGSTTMSGTSMASPHVAGMGAYMIGMGADPRQVCDRLKQLATAAIRNPGSSTTNR
                     LLYNGSGQ"
     misc_feature    8443..8467
                     /note="RB T-DNA repeat; right border repeat from nopaline 
                     C58 T-DNA"
     rep_origin      8606..9237
                     /note="oriV; incP origin of replication"