LOCUS       Exported                3772 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pND50 DNA, complete sequence.
ACCESSION   AB206288
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Yamagishi J, Kojima T, Oyamada Y, Fujimoto K, Hattori H, Nakamura S,
            Inoue M.
  TITLE     Alterations in the DNA topoisomerase IV grlA gene responsible for 
            quinolone resistance in Staphylococcus aureus
  JOURNAL   Antimicrob. Agents Chemother. 40 (5), 1157-1163 (1996)
  PUBMED    8723458
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Inoue R, Kaito C, Tanabe M, Kamura K, Akimitsu N, Sekimizu K.
  TITLE     Genetic identification of two distinct DNA polymerases, DnaE and 
            PolC, that are essential for chromosomal DNA replication in 
            Staphylococcus aureus
  JOURNAL   Mol. Genet. Genomics 266 (4), 564-571 (2001)
  PUBMED    11810227
REFERENCE   3  (bases 1 to 3772)
  AUTHORS   Matsuo M, Kurokawa K, Nishida S, Li Y, Takimura H, Kaito C, Fukuhara
            N, Maki H, Miura K, Murakami K, Sekimizu K.
  TITLE     Isolation and mutation site determination of the 
            temperature-sensitive murB mutants of Staphylococcus aureus
  JOURNAL   FEMS Microbiol. Lett. 222 (1), 107-113 (2003)
  PUBMED    12757953
REFERENCE   4  (bases 1 to 3772)
  AUTHORS   Kurokawa K, Matsuo M, Sekimizu K.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2005) Kenji Kurokawa, The University of Tokyo, 
            Graduate School of Pharmaceutical Sciences; 7-3-1, Hongo, Bunkyo-ku,
            Tokyo 113-0033, Japan (E-mail:kurokawa@mol.f.u-tokyo.ac.jp, 
            URL:http://www.f.u-tokyo.ac.jp/~hassei/, Tel:81-3-5841-4821, 
            Fax:81-3-5684-2973)
REFERENCE   5  (bases 1 to 3772)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Cloning vector pND50"
                     /mol_type="other DNA"
                     /note="A shuttle vector between Escherichia coli and 
                     Staphylococcus aureus. Harboring the chloramphenicol acetyl
                     transferase gene derived from pC194, a Staphylococcal 
                     plasmid replication origin from pUB110, an E. coli plasmid 
                     replication origin from pUC19, and a multi-cloning site 
                     from pUC18."
                     /db_xref="taxon:316466"
     misc_feature    1..66
                     /label=pUC18-derived sequence
                     /note="pUC18-derived sequence"
     misc_feature    1..57
                     /label=multi-cloning sites of pUC18
                     /note="multi-cloning sites of pUC18"
     misc_feature    67..908
                     /label=pUC19-derived sequence
                     /note="pUC19-derived sequence"
     rep_origin      264..852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    join(909..1327,2171..3772)
                     /label=pUB110-derived sequence
                     /note="pUB110-derived sequence"
     misc_feature    1328..2170
                     /label=pC194-derived sequence
                     /note="pC194-derived sequence"
     CDS             complement(1427..2077)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /function="chloramphenicol resistance"
                     /label=cat
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="BAD90928.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(1427..2077)
                     /gene="cat"
                     /label=cat
     CDS             complement(2356..3360)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             complement(2356..3063)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="putative pUB110-derived replication protein"
                     /protein_id="BAD90929.1"
                     /translation="MKHGIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSD
                     MAQGFRRMMQYKKINKNLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVN
                     QKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEK
                     NLKRLSDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIA
                     MWNWERKNYFIKE"