LOCUS       Exported                9154 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNB95, complete sequence.
ACCESSION   KT826601
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by 
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel 
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
  PUBMED    26636338
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460 
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Cloning vector pNB95"
                     /mol_type="other DNA"
                     /db_xref="taxon:1766774"
     CDS             458..1252
                     /codon_start=1
                     /gene="neo"
                     /product="neomycin phosphotransferase"
                     /label=neo
                     /protein_id="ALR84556.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            458..1252
                     /gene="neo"
                     /label=neo
     regulatory      1552..1762
                     /regulatory_class="promoter"
                     /note="nptII"
     CDS             1767..3524
                     /codon_start=1
                     /product="TonB dependent siderophore receptor"
                     /label=TonB dependent siderophore receptor
                     /protein_id="ALR84557.1"
                     /translation="MTLLPRETHDSGRHNPIVAPSTRRKRPLAAGLMSATACVALLHFV
                     SDARAQSVSASETSTIPVNAAKAPAKSKVKVNAQSRSTRARAVSAPVGDAPATSRPETI
                     MVTGSLFRDPNTTSASPITHITMKDMQQRGIKTVTDALQLLSSNGAGNLTNAWSAGGGF
                     AAGASAPSLRGLSTDSTLVLMDGQRLSYYPLADDGERNFVDTNWMPQSIMQGVDVLQDG
                     GSATYGADAVAGVVNMITRKEIKGFEGNAEGGLTQRGDAGHQRLYATWGHGDLHKDGYN
                     FYINSEYQQDDPLYYRQLNSPYNNGDLTGIGGSNGNDNIVSGGSITNFAPSTVALVRPV
                     TGTGTNTAPWSLLNQQAGCRGLDGLVTGTVGGSTGISQACSWNTQKYAQVAPDLRRVSA
                     TAHFTANVTERSQLTAMFTWSQARSIITGTPYSTYGGTSYSQAKLATAQNTYLPVYLAD
                     GSLNPNNPYAAQGEQAQVNYRFGDLIPTTMDTSQNFRGSVRYNGWHPSRWGSDWNYDVN
                     FVGMNTLLNQTITGVPTISGIQNAITSGSYNFVDPSQNSQSVLNAIAPRNVINARTQEY
                     SGEATLSKGL"
     CDS             3720..3749
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3774..5111
                     /codon_start=1
                     /product="alkaline phosphatase"
                     /label=alkaline phosphatase
                     /protein_id="ALR84558.1"
                     /translation="PVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLI
                     GDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATA
                     WSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRK
                     CYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLR
                     EQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVT
                     CTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVD
                     LDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYG
                     NSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
     rep_origin      6700..7362
     CDS             complement(6700..7362)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7363..8132
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"