LOCUS       Exported                5223 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pNAK1, complete sequence.
ACCESSION   AY827555
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Nakayama S, Irie R, Kosaka T, Matsuura K, Yoshino S, Furukawa K.
  TITLE     New host-vector system in solvent-producing Clostridium 
            saccharoperbutylacetonicum strain N1-4
  JOURNAL   J. Gen. Appl. Microbiol. 53 (1), 53-56 (2007)
  PUBMED    17429161
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Nakayama S, Kousaka T, Matsuura K, Yoshino S, Furukawa K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2004) Laboratory of Applied Microbiology, Kyushu 
            University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
REFERENCE   3  (bases 1 to 5223)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Shuttle vector pNAK1"
                     /mol_type="other DNA"
                     /db_xref="taxon:303680"
     promoter        6..24
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    37..145
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     61..77
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(154..172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             795..1265
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="AAV86077.1"
                     /translation="MLLTNKGVIGLSKEDRVLYAGSDEWVNTRTGETITANQIIKKTDR
                     NGFMITYLSAIINLIETLGNRKMQVVKYILENMDKSNNTLIITNRELAEKSKASLNTVV
                     KTIAILKKAKIITTRTGAIMITPELVHRGSKSKEQYLLTKFQEFEEVKSNEI"
     gene            795..1265
                     /gene="rep"
                     /label=rep
     CDS             complement(1323..2144)
                     /codon_start=1
                     /gene="mob"
                     /product="mobilization protein"
                     /label=mob
                     /protein_id="AAV86078.1"
                     /translation="MSIFKIRSVRKMWYQQWFTWTRPKG?GVAPHMHFSFVPMNEDGTL
                     SAKKKINRNFLREIQDEFPRYMKGYGFNVERGMENSTKKHLEPLEFKKQELKKDIENAK
                     QEQERFEKKYSNFKESIKTRAENLQKTSESLSKLEEHTKGVLGKLDKIQVKKGLFGDKL
                     TISEQDYSILVSLAKAGEGKLIENLQLRSKIGD?TQKLSKLDAINNEFQRLKGIELKHF
                     ELQRKYRNLNISFERVEKAINNLDLVEPVNKEIQSVKIAEMSISKSFDIER"
     gene            complement(1323..2144)
                     /gene="mob"
                     /label=mob
     CDS             3190..3849
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AAV86079.1"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            3190..3849
                     /gene="cat"
                     /label=cat
     terminator      4284..4370
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4433..5021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"