LOCUS       Exported               12530 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pMycoFos, complete sequence.
ACCESSION   HQ388459
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Ly MA, Liew EF, Le NB, Coleman NV.
  TITLE     Construction and evaluation of pMycoFos, a fosmid shuttle vector for
            Mycobacterium spp. with inducible gene expression and copy number 
            control
  JOURNAL   J. Microbiol. Methods 86 (3), 320-326 (2011)
  PUBMED    21689690
REFERENCE   2  (bases 1 to 12530)
  AUTHORS   Ly MA, Coleman NV.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2010) School of Molecular Bioscience, University 
            of Sydney, Maze Crescent, Darlington, NSW 2006, Australia
REFERENCE   3  (bases 1 to 12530)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Shuttle vector pMycoFos"
                     /mol_type="other DNA"
                     /label=Escherichia coli/Mycobacterium shuttle plasmid
                     /note="Escherichia coli/Mycobacterium shuttle plasmid"
                     /db_xref="taxon:930172"
     rep_origin      83..1979
                     /note="oriM; derived from pAL5000"
     CDS             complement(2030..3103)
                     /codon_start=1
                     /gene="amiC"
                     /product="AmiC"
                     /label=amiC
                     /note="acetamidase regulator"
                     /protein_id="ADR02799.1"
                     /translation="MQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ
                     GRKVTIEVLDGGRPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT
                     SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY
                     CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD
                     RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN
                     AMAESCYEGLVALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS
                     ADGYDFDILDTLTTLDA"
     gene            complement(2030..3103)
                     /gene="amiC"
                     /label=amiC
     CDS             3206..3634
                     /codon_start=1
                     /gene="amiA"
                     /product="AmiA"
                     /label=amiA
                     /note="acetamidase regulator"
                     /protein_id="ADR02800.1"
                     /translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE
                     LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL
                     RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS"
     gene            3206..3634
                     /gene="amiA"
                     /label=amiA
     CDS             3714..3998
                     /codon_start=1
                     /gene="amiD"
                     /product="AmiD"
                     /label=amiD
                     /note="acetamidase regulator"
                     /protein_id="ADR02801.1"
                     /translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL
                     TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY"
     gene            3714..3998
                     /gene="amiD"
                     /label=amiD
     CDS             4003..4644
                     /codon_start=1
                     /gene="amiS"
                     /product="AmiS"
                     /label=amiS
                     /note="acetamidase transporter"
                     /protein_id="ADR02802.1"
                     /translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT
                     VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH
                     AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV
                     SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAAPSVTNPPPAALAATTR"
     gene            4003..4644
                     /gene="amiS"
                     /label=amiS
     CDS             4802..5617
                     /codon_start=1
                     /product="AphA"
                     /function="kanamycin resistance"
                     /label=AphA
                     /note="glycoside-3-phosphotransferase"
                     /protein_id="ADR02803.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5757..6104
                     /codon_start=1
                     /gene="resD"
                     /product="ResD"
                     /label=resD
                     /note="F plasmid resolvase"
                     /protein_id="ADR02804.1"
                     /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP
                     VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE
                     SDAVAMLKQLS"
     gene            5757..6104
                     /gene="resD"
                     /label=resD
     rep_origin      6499..7113
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      6634..7140
                     /label=oriV
                     /note="oriV"
     rep_origin      7189..7408
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      7196..7412
                     /label=oriS
                     /note="oriS"
     CDS             7499..8254
                     /codon_start=1
                     /gene="repE"
                     /product="RepE"
                     /label=repE
                     /note="replication protein from F plasmid"
                     /protein_id="ADR02805.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            7499..8254
                     /gene="repE"
                     /label=repE
     misc_feature    8257..8507
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8833..10008
                     /codon_start=1
                     /gene="parA"
                     /product="ParA"
                     /label=parA
                     /note="plasmid partitioning protein"
                     /protein_id="ADR02806.1"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            8833..10008
                     /gene="parA"
                     /label=parA
     CDS             10008..10979
                     /codon_start=1
                     /gene="parB"
                     /product="ParB"
                     /label=parB
                     /note="plasmid partitioning protein"
                     /protein_id="ADR02807.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            10008..10979
                     /gene="parB"
                     /label=parB
     misc_feature    11052..11525
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     repeat_region   11075..11529
                     /label=parC region
                     /note="parC region"
     misc_feature    11784..12187
                     /label=lambda cosN site
                     /note="lambda cosN site"
     misc_feature    11785..12183
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_recomb     12199..12234
                     /label=loxP site
                     /note="loxP site"
     protein_bind    12201..12234
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."