LOCUS       Exported                5129 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter-trap vector pMW82, complete sequence.
ACCESSION   EF363313
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Bumann D.
  TITLE     In vivo visualization of bacterial colonization, antigen expression,
            and specific T-cell induction following oral administration of live 
            recombinant Salmonella enterica serovar Typhimurium
  JOURNAL   Infect. Immun. 69 (7), 4618-4626 (2001)
  PUBMED    11402006
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Rollenhagen C, Sorensen M, Rizos K, Hurvitz R, Bumann D.
  TITLE     Antigen selection based on expression levels during infection 
            facilitates vaccine development for an intracellular pathogen
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (23), 8739-8744 (2004)
  PUBMED    15173591
REFERENCE   3  (bases 1 to 5129)
  AUTHORS   Wendland M, Bumann D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2007) Molecular Biology, Max-Planck-Institute for 
            Infection Biology, Chartieplatz 1, Berlin D-10117, Germany
REFERENCE   4  (bases 1 to 5129)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Promoter-trap vector pMW82"
                     /mol_type="other DNA"
                     /db_xref="taxon:424500"
     CDS             32..823
                     /codon_start=1
                     /product="gfp/ova fusion"
                     /label=gfp/ova fusion
                     /note="fluorescent reporter; fusion of green-fluorescent 
                     protein to chicken ovalbumin, contains strong CD4 T cell 
                     epitope"
                     /protein_id="ABN41558.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKESLKISQAVHAAHAEINEAGREVVG"
     CDS             32..745
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1038..1124
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1216..1243
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1262..1353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1354..2214
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2385..2973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3159..3299
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3401..3592)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"