LOCUS       Exported                3675 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMVAX1(c), complete sequence.
ACCESSION   KF703899
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Du Y, Lu Y, Wang X, Qi J, Liu J, Hu Y, Li F, Wu J, Guo L, Liu J, Tao
            H, Sun W, Chen L, Cong X, Ren S, Shi J, Li J, Wang J, Huang B, Wan 
            R.
  TITLE     Highly Efficient Expression of Interleukin-2 under the Control of 
            Rabbit beta-Globin Intron II Gene Enhances Protective Immune 
            Responses of Porcine Reproductive and Respiratory Syndrome (PRRS) 
            DNA Vaccine in Pigs
  JOURNAL   PLoS ONE 9 (3), E90326 (2014)
  PUBMED    24603502
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Du Y, Wang X, Huang B, Liu J, Bi J, Wu X, Wu J, Guo L, Chen L, Sun 
            W, Wang J, Wan R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2013) Institute of Animal Science and Veterinary 
            Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road 
            No. 8, Jinan, Shandong 250100, China
REFERENCE   3  (bases 1 to 3675)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Expression vector pMVAX1(c)"
                     /mol_type="other DNA"
                     /db_xref="taxon:1436817"
     regulatory      33..757
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      758..1406
                     /regulatory_class="enhancer"
                     /note="rabbit beta-globin intron II"
     intron          781..1353
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        1408..1426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1409..1427
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     misc_feature    1429..1487
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     1499..1516
                     /label=BGH reverse priming site
                     /note="BGH reverse priming site"
     regulatory      1505..1729
                     /regulatory_class="polyA_signal_sequence"
                     /note="BGH polyadenylation signal"
     polyA_signal    1505..1729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             1902..2696
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /protein_id="AHC69779.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3022..3610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"