LOCUS       Exported               10047 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMV5 DNA, complete sequence.
ACCESSION   AB183144
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Inui M, Tsuge Y, Suzuki N, Vertes AA, Yukawa H.
  TITLE     Isolation and characterization of a native composite transposon, 
            Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum 
            chromosomal DNA
  JOURNAL   Appl. Environ. Microbiol. 71 (1), 407-416 (2005)
  PUBMED    15640215
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2004) Masayuki Inui, Research Institute of 
            Innovative Technology for the Earth (RITE), Microbiology Research 
            Group; 9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan 
            (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax:81-774-75-2321)
REFERENCE   3  (bases 1 to 10047)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Cloning vector pMV5"
                     /mol_type="other DNA"
                     /db_xref="taxon:283954"
     CDS             870..2357
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /protein_id="BAD83816.1"
                     /translation="MYMIIPNSTPAYPISGSRVNTPSRLRPDCAGLDDTPASTVDRDKL
                     LAHLGRTALHGSISRNFKGAYRLVVDKETGEKRSVPNLYRIDSEKLGRCEYVMLTSKQY
                     ASVMVIDVDQIGEAGGHPENLNSYVKGVIWVLVQHGIGPAWAGINPISGKAQFIWLIDP
                     VYAGKNRASRNMELLKATSHELGELLDHDPHFAHRFSRSPFYTGKSPEAYRWYCQHDRV
                     IRLQDFLRQVREMAGQSQHIKNKRQQFNSGRELINAVKTRREEAQAFKALAEDVENEIS
                     EEIDQYDPELIDGVRVRWISQGVAARDETAFSHALKIGHRLRKAGQRLTDAAVIDAYEH
                     AYNVAQQQGSAGRDSEMPPMRDRQTMARRVRGYVTQSKTNTSLGASAPGRVTSSERKAL
                     ATMGRKGGQKAAQRWKTDPEGQYAQNQLQKLKKTHRKKRVEGQTTRAKIQALIGEAYVQ
                     TGEVLTRKQIVDETGLSRATVTRHLAALREQGALPET"
     gene            870..2357
                     /gene="repA"
                     /label=repA
     regulatory      2067..2076
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(4213..4229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4237..4253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4261..4291)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4306..4327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4615..5203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5692..5708
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5749..6194
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             6046..6096
                     /codon_start=1
                     /gene="sacR"
                     /product="SacR"
                     /label=sacR
                     /protein_id="BAD83817.1"
                     /translation="MCKGQSVYFGVTPYIF"
     gene            6046..6096
                     /gene="sacR"
                     /label=sacR
     CDS             6195..7616
                     /codon_start=1
                     /gene="sacB"
                     /product="levansucrase"
                     /label=sacB
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="BAD83818.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            6195..7616
                     /gene="sacB"
                     /label=sacB
     CDS             7823..8605
                     /codon_start=1
                     /gene="spc"
                     /product="spectinomycin adenyltransferase"
                     /label=spc
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="BAD83819.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            7823..8605
                     /gene="spc"
                     /label=spc
     mobile_element  8764..9531
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     primer_bind     10005..10021
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"