LOCUS       Exported                5693 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pMV261-EO6, complete sequence.
ACCESSION   DQ115380
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Shuttle vector pMV261-EO6"
                     /mol_type="other DNA"
                     /note="Mycobacterium-E. coli shuttle vector; murine EO 
                     rotavirus VP6 of GenBank Accession Number AY947543 cloned 
                     into BamH1-Hind111 sites of pMV261 (Stover et al., 1991)"
                     /db_xref="taxon:336698"
     misc_feature    6..1218
                     /note="APH1 region of Tn903 encoding 
                     aminoglycoside-3-phosphotransferase, conferring kanamycin 
                     resistance"
     CDS             120..935
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1223..2009
                     /label=OriE region derived from pUC19
                     /note="OriE region derived from pUC19"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2028..3927
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3950..4335
                     /regulatory_class="promoter"
                     /note="derived from Mycobacterium bovis hsp60 promoter of 
                     GenBank Accession Number M17705"
     CDS             4335..5555
                     /codon_start=1
                     /product="Hsp60/VP6 chimera"
                     /label=Hsp60/VP6 chimera
                     /note="first six amino acids of Mycobacterium bovis hsp60 
                     linked to 397 amino acids of EO rotavirus VP6"
                     /protein_id="AAZ16498.1"
                     /translation="MAKTIADPKMDVLYSISRTLKDARDKIVEGTLYSNVSDLIQQFNQ
                     MLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLNLDANYVESARTTIDYFVDFIDNV
                     CMDEMVRESQRNGIAPQSDALRKLSGVKFRRINFNNSSEYIENWNLQNRRQRTGFTFHK
                     PNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQVAGFDYSCAINAPANIQQFEHIVQ
                     LRRVLTTATITLLPDAERFSFPRVINSADGATTWYFNPVILRPNNVEVEFLLNGQIINT
                     YQARFGTIVARNFDTIRLSFQLMRPPNMTPAVAALFPNAQPFEHHATVGLTLRIDSAIC
                     ESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWTDLITNYSPSREDNLQRVFTVAS
                     IRSMLVK"
     terminator      5611..5657
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      5614..5657
                     /regulatory_class="terminator"
                     /note="rrnBT1"