LOCUS       Exported                9445 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMU125, complete sequence.
ACCESSION   KJ955629
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Bahl CD.
  TITLE     Sequencing commonly used, cryptic plasmids from Acinetobacter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Bahl CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2014) Biochemistry, University of Washington, Box 
            351655, Room 420, Seattle, WA 98195-1655, USA
REFERENCE   3  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Cloning vector pMU125"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:1544017"
     misc_feature    110..250
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      1619..2955
                     /label=pWH1266_ori
                     /note="pWH1266_ori"
     CDS             complement(5732..6400)
                     /codon_start=1
                     /product="TetA"
                     /label=TetA
                     /note="class C tetracycline resistance protein"
                     /protein_id="AIO09752.1"
                     /translation="MSRPAGIRYQAYRYRRPMPLRAFNPVSSFRWARGMTIVAALMTVF
                     FIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQ
                     AIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQ
                     GSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
                     "
     CDS             complement(6401..7117)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /protein_id="AIO09750.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(7486..7514)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        7627..7731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7732..8592
                     /codon_start=1
                     /product="beta-lactamase TEM"
                     /label=beta-lactamase TEM
                     /protein_id="AIO09751.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8747..9366
                     /label=pBR322_ori
                     /note="pBR322_ori"
     rep_origin      8763..9351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"