LOCUS       Exported                3568 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMU102, complete sequence.
ACCESSION   JF423903
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Computational Design and Characterization of a Temperature-sensitive
            Replicon for Gram Positive Thermophiles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) Thayer School of Engineering, Dartmouth 
            College, 8000 Cummings, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 3568)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Expression vector pMU102"
                     /mol_type="other DNA"
                     /db_xref="taxon:1077343"
     rep_origin      1..589
                     /note="Escherichia coli origin of replication derived from 
                     pUC19"
     CDS             1281..2285
                     /codon_start=1
                     /product="RepB"
                     /label=RepB
                     /note="replication protein"
                     /protein_id="AEN69097.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             2392..3042
                     /codon_start=1
                     /product="Cat"
                     /label=Cat
                     /note="chloramphenicol acetyltransferase; provides 
                     resistance to thiamphenicol"
                     /protein_id="AEN69098.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     protein_bind    3172..3193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3208..3238
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3246..3262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3270..3286
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"