LOCUS       Exported                5086 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Positive selection vector pMTet1, complete sequence.
ACCESSION   AF438204
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Matin MM, Hornby DP.
  TITLE     A positive selection vector combining tetracycline resistance that 
            eliminates the need for bacterial plating
  JOURNAL   Anal. Biochem. 278 (1), 46-51 (2000)
  PUBMED    10640352
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Matin MM, Hornby DP.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2001) Molecular Biology, Krebs Institute, Firth 
            Court, Western Bank, Sheffield S10 2TN, UK
REFERENCE   3  (bases 1 to 5086)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Positive selection vector pMTet1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:177164"
     CDS             92..1282
                     /codon_start=1
                     /gene="tetA(C)"
                     /product="tetracycline resistance protein"
                     /label=tetA(C)
                     /note="modified form of the pBR322 tetA(C)"
                     /protein_id="AAL31631.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            92..1282
                     /gene="tetA(C)"
                     /label=tetA(C)
     rep_origin      2799..3387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3675..3696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3711..3741
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3749..3765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3773..3789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3829..5085
                     /codon_start=1
                     /product="C5 DNA methyltransferase"
                     /label=C5 DNA methyltransferase
                     /note="from M.MspIm"
                     /protein_id="AAL31632.1"
                     /translation="MKPEILKLIRSKLDLTQKQASEIIEVSDKAWQQWESGKTEMHPAY
                     YSFLQEKLKDKINFEELSAQKTLQKKIFDKYNQNQITKNAEELAEITHIEERKDAYSSD
                     FKFIDLFSGIGGIRQSFEVNGGKCVFSSEIDPFAKFTYYTNFGVVPFGDITKVEATTIP
                     EHDILCAGFPGQPWSHMGKREGFEHPTQGTMFHEIVRIIETKKTPVLFLENVPGLINHD
                     DGNTLKVIIETLEDMGYKVHHTVLDASHFGIPQKRKRFYLVAFLNQNIHFEFPKPPMIS
                     KDIGEVLESDVTGYSISEHLQKSYLFKKDDGKPSLIDKNTTGAVKTLVSTYHKIQRLTG
                     TFVKDGETAIRLLTTNECKAIMGFPKDFVIPVSRTQMYRQMGNSVVVPVVTKIAEQISL
                     ALKTVNQQSPQENFELELV"