LOCUS       Exported                5702 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Gene silencing vector pMTCbLCVA.008, complete sequence.
ACCESSION   AY279346
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Geminivirus-based vectors for gene silencing in Arabidopsis
  JOURNAL   Plant J. 30 (1), 107-114 (2002)
  PUBMED    11967097
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2003) Botany, North Carolina State University, Box
            7612, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Gene silencing vector pMTCbLCVA.008"
                     /lab_host="Arabidopsis thaliana"
                     /mol_type="other DNA"
                     /note="geminivirus-based silencing vector; contains 360 bp 
                     Arabidsopsis ChlI coding sequence"
                     /db_xref="taxon:228590"
     promoter        complement(859..963)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(989..1444)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     1586..1602
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1612..1630
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1640..4452
                     /label=derived from DNA A of Cabbage leaf curl virus
                     /note="derived from DNA A of Cabbage leaf curl virus"
     misc_feature    3652..4024
                     /note="derived from GenBank Accession Number AY140022; 360 
                     bp of Arabidopsis thaliana ChlI replaces Cabbage leaf curl 
                     virus coat protein"
     promoter        complement(4500..4518)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4539..4555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4563..4579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4587..4617)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4632..4653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4941..5529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5700..858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"