LOCUS       Exported                8721 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pmT8 DNA, complete sequence.
ACCESSION   AB551244
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Kuroda M, Kimizu M, Mikami C.
  TITLE     A simple set of plasmids for the production of transgenic plants
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (11), 2348-2351 (2010)
  PUBMED    21071849
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Kuroda M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2010) Contact:Masaharu Kuroda National 
            Agricultural Research Center, Rice Physiology Research Team; Inada 
            1-2-1, Joetsu, Niigata 943-0193, Japan
REFERENCE   3  (bases 1 to 8721)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Binary vector pmT8"
                     /mol_type="other DNA"
                     /db_xref="taxon:751898"
     misc_feature    39..63
                     /label=T-DNA right border
                     /note="T-DNA right border"
     CDS             complement(1610..2260)
                     /codon_start=1
                     /gene="tetR"
                     /label=tetR
                     /inference="similar to DNA sequence:same species:X75761.1"
                     /protein_id="BAJ22055.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(1610..2260)
                     /gene="tetR"
                     /label=tetR
     CDS             2366..3565
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             2795..3565
                     /codon_start=1
                     /gene="tetA"
                     /label=tetA
                     /inference="similar to DNA sequence:same species:X75761.1"
                     /protein_id="BAJ22056.1"
                     /translation="MVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGE
                     RRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATT
                     IGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPI
                     MVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITT
                     WNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR"
     gene            2795..3565
                     /gene="tetA"
                     /label=tetA
     misc_feature    complement(4795..6170)
                     /gene="trfA"
                     /inference="similar to DNA sequence:same species:X00713.1"
     gene            complement(4795..6170)
                     /gene="trfA"
                     /label=trfA
     CDS             complement(4838..5986)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6258..6629)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6662..6771
                     /note="incP origin of transfer"
     rep_origin      6924..7555
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      6932..7549
                     /label=derived from plasmid RK2
                     /note="derived from plasmid RK2"
                     /inference="similar to DNA sequence:same species:M20134.1"
     misc_feature    7918..7942
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    7920..7944
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    8656..8712
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"