LOCUS       Exported                5908 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pMT2-PL, complete sequence.
ACCESSION   KX757040
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Bair CR, Kotha Lakshmi Narayan P, Kajon AE.
  TITLE     The Adenovirus Tripartite Leader Sequence Enables Efficient Ectopic 
            Expression of Human Adenovirus E3 CR1 Transcripts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Bair CR, Kotha Lakshmi Narayan P, Lamson DM, Kajon AE.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2016) Infectous Disease Program, Lovelace 
            Respiratory Research Institute, 2425 Ridgecrest Drive SE, 
            Albuquerque, NM 87108, USA
REFERENCE   3  (bases 1 to 5908)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 8.1.7
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Mammalian expression vector pMT2-PL"
                     /mol_type="other DNA"
                     /db_xref="taxon:1966386"
     misc_feature    1..65
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     regulatory      95..232
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    95..229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(627..1718)
                     /codon_start=1
                     /gene="ada"
                     /product="adenosine deaminase"
                     /label=ada
                     /protein_id="AQX83303.1"
                     /translation="MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEG
                     LLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSP
                     HLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSP
                     KVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAE
                     VVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVI
                     RLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEK
                     RELLDLLYKAYGMPPSASAGQNL"
     gene            complement(627..1718)
                     /gene="ada"
                     /label=ada
     regulatory      complement(1793..1995)
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(1799..2128)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1813..1948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(1871..1948)
                     /direction=LEFT
                     /label=SV40 minimum origin of replication
                     /note="SV40 minimum origin of replication"
     misc_feature    2138..2371
                     /label=VARNA
                     /note="VARNA"
     rep_origin      complement(2608..3227)
                     /direction=LEFT
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      complement(2623..3211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3382..4242)
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /note="ampicillin resistance protein"
                     /protein_id="AQX83304.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3382..4242)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(4243..4347)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4821..4837
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4847..5176)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4861..4996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5204..5504
                     /regulatory_class="promoter"
                     /note="human adenovirus type 2 major late promoter"
     misc_feature    5505..5636
                     /note="leader 2 and leader 3 from the human adenovirus type
                     2 tripartite leader sequence"
     intron          5637..5900
                     /note="synthetic intron"
     intron          5645..5874
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"