LOCUS       Exported               11397 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMON93914, complete sequence.
ACCESSION   JN400383
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil 
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R, 
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean 
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
  PUBMED    22363475
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2011) Yield & Stress Traits, Monsanto Company, 700
            Chesterfield Parkway West, Chesterfield, MO 63017, USA
REFERENCE   3  (bases 1 to 11397)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Expression vector pMON93914"
                     /mol_type="other DNA"
                     /db_xref="taxon:1089336"
     regulatory      25..637
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     promoter        295..639
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    680..1164
                     /label=derived from G. max BBX52 suppression element
                     /note="derived from G. max BBX52 suppression element"
     3UTR           1203..1638
                     /label=derived from G. barbadense E6
                     /note="derived from G. barbadense E6"
     misc_feature    complement(1675..2116)
                     /label=derived from A. tumifaciens T-DNA left border
                     /note="derived from A. tumifaciens T-DNA left border"
     misc_feature    1937..1961
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     CDS             3337..3528
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    3630..3770
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3956..4544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5075..5963
                     /label=derived from E. coli aadA
                     /note="derived from E. coli aadA"
     CDS             5117..5905
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    complement(6059..6469)
                     /label=derived from A. tumifaciens T-DNA left border
                     /note="derived from A. tumifaciens T-DNA left border"
     misc_feature    6214..6238
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     3UTR           complement(6484..7126)
                     /label=derived from P. sativum RbcS2
                     /note="derived from P. sativum RbcS2"
     terminator      6485..7126
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(7133..8500)
                     /codon_start=1
                     /product="CP4 EPSPS"
                     /label=CP4 EPSPS
                     /note="derived from A. tumifaciens aroA-CP4"
                     /protein_id="AEP17826.1"
                     /translation="MLHGASSRPATARKSSGLSGTVRIPGDKSISHRSFMFGGLASGET
                     RITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLLAPEAPLDFGNAATGCR
                     LTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKSEDGDRLPVTLRGPKTPT
                     PITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEKMLQGFGANLTVETDADG
                     VRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVTILNVLMNPTRTGLILTL
                     QEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPSMIDEYPILAVAAAFAEG
                     ATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVRGRPDGKGLGNASGAAVA
                     THLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMAGLGAKIELSDTKAA"
     CDS             complement(8501..8728)
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label=EPSPS CTP
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17827.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     5UTR           complement(8738..9405)
                     /label=derived from A. thaliana Tsf1
                     /note="derived from A. thaliana Tsf1"
     regulatory      complement(9406..9885)
                     /regulatory_class="promoter"
                     /note="derived from A. thaliana Tsf1"
     regulatory      complement(9909..10445)
                     /regulatory_class="enhancer"
                     /note="FMV 35S"
     misc_feature    complement(10468..10824)
                     /label=derived from A. tumifaciens T-DNA right border
                     /note="derived from A. tumifaciens T-DNA right border"
     misc_feature    10508..10532
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    10996..11352
                     /label=derived from A. tumifaciens T-DNA right border
                     /note="derived from A. tumifaciens T-DNA right border"
     misc_feature    11288..11312
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"