LOCUS       Exported               10411 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMON81312, complete sequence.
ACCESSION   JN400384
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil 
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R, 
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean 
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
  PUBMED    22363475
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2011) Yield & Stress Traits, Monsanto Company, 700
            Chesterfield Parkway West, Chesterfield, MO 63017, USA
REFERENCE   3  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Expression vector pMON81312"
                     /mol_type="other DNA"
                     /db_xref="taxon:1089334"
     misc_feature    complement(1..442)
                     /label=derived from A. tumifaciens T-DNA left border
                     /note="derived from A. tumifaciens T-DNA left border"
     misc_feature    156..180
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      481..1080
                     /regulatory_class="promoter"
                     /note="derived from A. thaliana Act7"
     5UTR           1081..1718
                     /label=derived from A. thaliana Act7
                     /note="derived from A. thaliana Act7"
     CDS             1721..1948
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label=EPSPS CTP
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17828.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             1949..3316
                     /codon_start=1
                     /product="CP4 EPSPS"
                     /label=CP4 EPSPS
                     /note="derived from A. tumifaciens aroA-CP4"
                     /protein_id="AEP17829.1"
                     /translation="MLHGASSRPATARKSSGLSGTVRIPGDKSISHRSFMFGGLASGET
                     RITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLLAPEAPLDFGNAATGCR
                     LTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKSEDGDRLPVTLRGPKTPT
                     PITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEKMLQGFGANLTVETDADG
                     VRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVTILNVLMNPTRTGLILTL
                     QEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPSMIDEYPILAVAAAFAEG
                     ATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVRGRPDGKGLGNASGAAVA
                     THLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMAGLGAKIELSDTKAA"
     3UTR           3323..3575
                     /label=derived from A. tumifaciens nos
                     /note="derived from A. tumifaciens nos"
     terminator      3323..3575
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3627..4239
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     promoter        3897..4241
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      4258..4267
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4264..4941
                     /codon_start=1
                     /product="B-box 32 protein"
                     /label=B-box 32 protein
                     /note="derived from A. thaliana BBX32"
                     /protein_id="AEP17830.1"
                     /translation="MVSFCELCGAEADLHCAADSAFLCRSCDAKFHASNFLFARHFRRV
                     ICPNCKSLTQNFVSGPLLPWPPRTTCCSESSSSSCCSSLDCVSSSELSSTTRDVNRARG
                     RENRVNAKAVAVTVADGIFVNWCGKLGLNRDLTNAVVSYASLALAVETRPRATKRVFLA
                     AAFWFGVKNTTTWQNLKKVEDVTGVSAGMIRAVESKLARAMTQQLRRWRVDSEEGWAEN
                     DNV"
     3UTR           4956..5270
                     /label=derived from G. barbadense E6
                     /note="derived from G. barbadense E6"
     misc_feature    5301..5657
                     /label=derived from A. tumifaciens T-DNA right border
                     /note="derived from A. tumifaciens T-DNA right border"
     misc_feature    5341..5365
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5794..6682
                     /label=derived from E. coli aadA
                     /note="derived from E. coli aadA"
     CDS             complement(5852..6640)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7213..7801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7987..8127
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(8229..8420)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      9709..10323
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      9800..10331
                     /label=oriV
                     /note="incP origin of replication"