LOCUS       Exported                9754 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMON74532, complete sequence.
ACCESSION   JN400386
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil 
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R, 
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean 
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
  PUBMED    22363475
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2011) Yield & Stress Traits, Monsanto Company, 700
            Chesterfield Parkway West, Chesterfield, MO 63017, USA
REFERENCE   3  (bases 1 to 9754)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Expression vector pMON74532"
                     /mol_type="other DNA"
                     /db_xref="taxon:1089333"
     misc_feature    complement(11..367)
                     /label=derived from A. tumifaciens T-DNA right border
                     /note="derived from A. tumifaciens T-DNA right border"
     misc_feature    51..75
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(504..1392)
                     /label=derived from E. coli aadA
                     /note="derived from E. coli aadA"
     CDS             complement(562..1350)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1923..2511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2697..2837
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2939..3130)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      4419..5033
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4510..5041
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    complement(5122..5563)
                     /label=derived from A. tumifaciens T-DNA left border
                     /note="derived from A. tumifaciens T-DNA left border"
     misc_feature    5277..5301
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      5602..6201
                     /regulatory_class="promoter"
                     /note="derived from A. thaliana Act7"
     5UTR           6202..6839
                     /label=derived from A. thaliana Act7
                     /note="derived from A. thaliana Act7"
     CDS             6842..7069
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label=EPSPS CTP
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17833.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             7070..8437
                     /codon_start=1
                     /product="CP4 EPSPS"
                     /label=CP4 EPSPS
                     /note="derived from A. tumifaciens aroA-CP4"
                     /protein_id="AEP17834.1"
                     /translation="MLHGASSRPATARKSSGLSGTVRIPGDKSISHRSFMFGGLASGET
                     RITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLLAPEAPLDFGNAATGCR
                     LTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKSEDGDRLPVTLRGPKTPT
                     PITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEKMLQGFGANLTVETDADG
                     VRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVTILNVLMNPTRTGLILTL
                     QEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPSMIDEYPILAVAAAFAEG
                     ATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVRGRPDGKGLGNASGAAVA
                     THLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMAGLGAKIELSDTKAA"
     3UTR           8444..8696
                     /label=derived from A. tumifaciens nos
                     /note="derived from A. tumifaciens nos"
     terminator      8444..8696
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      8748..9360
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     promoter        9018..9362
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     3UTR           9420..9734
                     /label=derived from G. barbadense E6
                     /note="derived from G. barbadense E6"